PMID- 19551289
OWN - NLM
STAT- MEDLINE
DCOM- 20090828
LR  - 20190608
IS  - 1678-9946 (Electronic)
IS  - 0036-4665 (Linking)
VI  - 51
IP  - 3
DP  - 2009 May-Jun
TI  - Spleen cell proliferation during and after skin myiasis by human bot fly 
      Dermatobia hominis.
PG  - 149-54
LID - S0036-46652009000300005 [pii]
AB  - Spleen cells from mice were examined at 5, 10, 15, 20 and 25 days post-infection 
      (dpi) with Dermatobia hominis larva and at 5, 10, 15, 30 and 60 days post-larval 
      emergence (dple). Cell proliferation in vitro assays were carried out with 
      RPMI-1640 medium and larval secretory product (LSP) of D. hominis at 5, 10, 15, 
      20 and 25 days. When each group of mice was tested against each medium, 
      significance was only seen for 25 dpi, with increasing order: LSP-10 d, -25 d, -5 
      d, -20 d, -15 d and RPMI. Significant results were also observed when each medium 
      was tested against mice at each dpi or dple. Each dple group vs. each medium 
      produced significant results only for 10 dple, with increasing order: LSP-5 d, 
      -20 d, -25 d, -10 d, -15 d and RPMI. Comparative tests were also carried out 
      between groups to refine certain observations. The LSPs were also analyzed using 
      SDS-PAGE. The results prove that myiasis caused depletion of spleen cells, 
      particularly under the effect of the LSP-10 and -15, but the cells tended to 
      increase up to 60 dple. This in vitro assay may represent the real systemic 
      immune response in the relationship LSP-D. hominis-host.
FAU - Goncalves, Jomara Mendes
AU  - Goncalves JM
AD  - Departamento de Parasitologia, Instituto de Ciencias Biologicas, UFMG, Belo 
      Horizonte, MG, Brazil.
FAU - Nascimento, Maria Fernanda Alves do
AU  - Nascimento MF
FAU - Breyner, Natalia Martins
AU  - Breyner NM
FAU - Fernandes, Viviane Cristina
AU  - Fernandes VC
FAU - Goes, Alfredo Miranda de
AU  - Goes AM
FAU - Leite, Antonio Cesar Rios
AU  - Leite AC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Brazil
TA  - Rev Inst Med Trop Sao Paulo
JT  - Revista do Instituto de Medicina Tropical de Sao Paulo
JID - 7507484
SB  - IM
MH  - Animals
MH  - *Cell Proliferation
MH  - Diptera
MH  - Larva
MH  - Male
MH  - Mice
MH  - Myiasis/immunology/*pathology
MH  - Skin/*parasitology/pathology
MH  - Spleen/immunology/*pathology
MH  - Time Factors
EDAT- 2009/06/25 09:00
MHDA- 2009/08/29 09:00
CRDT- 2009/06/25 09:00
PHST- 2009/02/03 00:00 [received]
PHST- 2009/04/13 00:00 [accepted]
PHST- 2009/06/25 09:00 [entrez]
PHST- 2009/06/25 09:00 [pubmed]
PHST- 2009/08/29 09:00 [medline]
AID - S0036-46652009000300005 [pii]
AID - 10.1590/s0036-46652009000300005 [doi]
PST - ppublish
SO  - Rev Inst Med Trop Sao Paulo. 2009 May-Jun;51(3):149-54. doi: 
      10.1590/s0036-46652009000300005.