PMID- 19553004
OWN - NLM
STAT- MEDLINE
DCOM- 20100219
LR  - 20100201
IS  - 1872-7980 (Electronic)
IS  - 0304-3835 (Linking)
VI  - 287
IP  - 1
DP  - 2010 Jan 1
TI  - PCC and COBRA-FISH a new tool to characterize primary cervical carcinomas: to 
      assess hall-marks and stage specificity.
PG  - 67-74
LID - 10.1016/j.canlet.2009.05.034 [doi]
AB  - A newly developed assay based on chemically induced premature chromosome 
      condensation (PCC) and multi-color combined binary ratio labeling (COBRA) 
      fluorescence in situ hybridization (FISH) techniques have been implemented in 
      order to investigate for the first time for recurrent cytogenetic aberrations in 
      primary cervical carcinoma (derived directly from biopsies) at different stages 
      of progression. The cytogenetic profiles of 17 biopsies derived from 14 and 3 
      cervical cancer patients with squamous-cell carcinomas (Sq) and with 
      adenocarcinomas (Ad), respectively, were assessed. Frequencies of both structural 
      as well as numerical aberrations were found to be higher in Sq than in Ad. The 
      analysis revealed that even in early tumors stages (IB1) have a higher frequency 
      of chromosome-losses and -gains as well as chromosomal alterations as compared to 
      normal cells. A positive trend was found between stage advancement of cervical 
      tumors and the frequency of numerical and structural aberrations. No specific and 
      common chromosomal abnormality (e.g. distinct clones of translocation) was found 
      among cervical carcinoma at the different stages (IB1, IIA and IIB). However, a 
      distinct difference was found between stage IIIB and lower tumor stages, as all 
      analyzed IIIB samples revealed a near tetraploid karyotype. Furthermore, all 
      studied metaphases were aberrant and had a high frequency of translocations. 
      PCC-COBRA-FISH characterization of a common type of an established culture from 
      cervical carcinoma CSCC-1 revealed a triploidy/tetraploidy karyotype with several 
      structural aberrations. In general, no similarity was found between this model 
      and early stages of primary tumors. The newly established assay has a novel 
      potential and can reveal the original status of primary tumors at different 
      stages.
CI  - Crown Copyright 2009. Published by Elsevier Ireland Ltd. All rights reserved.
FAU - Darroudi, Firouz
AU  - Darroudi F
AD  - Department of Toxicogenetics, Leiden University Medical Center, Einthovenweg 20, 
      2300RC Leiden, The Netherlands. F.Darroudi@LUMC.NL
FAU - Bergs, Judith W J
AU  - Bergs JW
FAU - Bezrookove, Vladimir
AU  - Bezrookove V
FAU - Buist, Marrije R
AU  - Buist MR
FAU - Stalpers, Lukas J
AU  - Stalpers LJ
FAU - Franken, Nicolaas A P
AU  - Franken NA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090623
PL  - Ireland
TA  - Cancer Lett
JT  - Cancer letters
JID - 7600053
SB  - IM
MH  - Adenocarcinoma/*genetics
MH  - Carcinoma, Squamous Cell/*genetics
MH  - Cell Line, Tumor
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human/*metabolism
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Neoplasm Staging
MH  - Uterine Cervical Neoplasms/*genetics/pathology
EDAT- 2009/06/26 09:00
MHDA- 2010/02/20 06:00
CRDT- 2009/06/26 09:00
PHST- 2008/08/20 00:00 [received]
PHST- 2009/05/27 00:00 [revised]
PHST- 2009/05/29 00:00 [accepted]
PHST- 2009/06/26 09:00 [entrez]
PHST- 2009/06/26 09:00 [pubmed]
PHST- 2010/02/20 06:00 [medline]
AID - S0304-3835(09)00404-2 [pii]
AID - 10.1016/j.canlet.2009.05.034 [doi]
PST - ppublish
SO  - Cancer Lett. 2010 Jan 1;287(1):67-74. doi: 10.1016/j.canlet.2009.05.034. Epub 
      2009 Jun 23.