PMID- 19553330
OWN - NLM
STAT- MEDLINE
DCOM- 20090831
LR  - 20211020
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 83
IP  - 17
DP  - 2009 Sep
TI  - Dexamethasone treatment of calves latently infected with bovine herpesvirus 1 
      leads to activation of the bICP0 early promoter, in part by the cellular 
      transcription factor C/EBP-alpha.
PG  - 8800-9
LID - 10.1128/JVI.01009-09 [doi]
AB  - Sensory neurons within trigeminal ganglia (TG) are the primary site for bovine 
      herpesvirus 1 (BHV-1) latency. During latency, viral gene expression is 
      restricted to the latency-related (LR) gene and the open reading frame ORF-E. We 
      previously constructed an LR mutant virus that expresses LR RNA but not any of 
      the known LR proteins. In contrast to calves latently infected with wild-type 
      (wt) BHV-1 or the LR rescued virus, the LR mutant virus does not reactivate from 
      latency following dexamethasone (DEX) treatment. In this study, we demonstrated 
      that bICP0, but not bICP4, transcripts were consistently detected in TG of calves 
      infected with the LR mutant or LR rescued virus following DEX treatment. Calves 
      latently infected with the LR rescued virus but not the LR mutant virus expressed 
      late transcripts, which correlated with shedding of infectious virus following 
      DEX treatment. The bICP4 and bICP0 genes share a common immediate-early promoter, 
      suggesting that this promoter was not consistently activated during DEX-induced 
      reactivation from latency. The bICP0 gene also contains a novel early promoter 
      that was activated by DEX in mouse neuroblastoma cells. Expression of a cellular 
      transcription factor, C/EBP-alpha, was stimulated by DEX, and C/EBP-alpha 
      expression was necessary for DEX induction of bICP0 early promoter activity. 
      C/EBP-alpha directly interacted with bICP0 early promoter sequences that were 
      necessary for trans activation by C/EBP-alpha. In summary, DEX treatment of 
      latently infected calves induced cellular factors that stimulated bICP0 early 
      promoter activity. Activation of bICP0 early promoter activity does not 
      necessarily lead to late gene expression and virus shedding.
FAU - Workman, Aspen
AU  - Workman A
AD  - Department of Veterinary and Biomedical Sciences, Nebraska Center for Virology, 
      University of Nebraska-Lincoln, Fair Street at East Campus Loop, Lincoln, 
      Nebraska 68583-0905, USA.
FAU - Perez, Sandra
AU  - Perez S
FAU - Doster, Alan
AU  - Doster A
FAU - Jones, Clinton
AU  - Jones C
LA  - eng
GR  - 1P20RR15635/RR/NCRR NIH HHS/United States
GR  - T32 AI060547/AI/NIAID NIH HHS/United States
GR  - R21AI069176/AI/NIAID NIH HHS/United States
GR  - R21 AI069176/AI/NIAID NIH HHS/United States
GR  - 1 T32 AIO60547/PHS HHS/United States
GR  - P20 RR015635/RR/NCRR NIH HHS/United States
GR  - P20 RR016469/RR/NCRR NIH HHS/United States
GR  - P20 RRO 16469/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20090624
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (CCAAT-Enhancer-Binding Protein-alpha)
RN  - 0 (DNA, Viral)
RN  - 0 (Trans-Activators)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
RN  - EC 2.3.2.27 (bICP0 protein, Bovine herpesvirus 1)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*administration & dosage
MH  - CCAAT-Enhancer-Binding Protein-alpha/*biosynthesis
MH  - Cattle
MH  - Cell Line
MH  - DNA, Viral/*metabolism
MH  - Dexamethasone/*administration & dosage
MH  - Herpesviridae Infections/*virology
MH  - Herpesvirus 1, Bovine/*physiology
MH  - Mice
MH  - Promoter Regions, Genetic
MH  - Protein Binding
MH  - Trans-Activators/*biosynthesis
MH  - Ubiquitin-Protein Ligases/*biosynthesis
MH  - *Virus Activation
PMC - PMC2738173
EDAT- 2009/06/26 09:00
MHDA- 2009/09/01 06:00
PMCR- 2010/03/01
CRDT- 2009/06/26 09:00
PHST- 2009/06/26 09:00 [entrez]
PHST- 2009/06/26 09:00 [pubmed]
PHST- 2009/09/01 06:00 [medline]
PHST- 2010/03/01 00:00 [pmc-release]
AID - JVI.01009-09 [pii]
AID - 1009-09 [pii]
AID - 10.1128/JVI.01009-09 [doi]
PST - ppublish
SO  - J Virol. 2009 Sep;83(17):8800-9. doi: 10.1128/JVI.01009-09. Epub 2009 Jun 24.