PMID- 19553558
OWN - NLM
STAT- MEDLINE
DCOM- 20090928
LR  - 20211020
IS  - 1556-679X (Electronic)
IS  - 1556-6811 (Print)
IS  - 1556-679X (Linking)
VI  - 16
IP  - 8
DP  - 2009 Aug
TI  - Autoimmune type 1 diabetes genetic susceptibility encoded by human leukocyte 
      antigen DRB1 and DQB1 genes in Tunisia.
PG  - 1146-50
LID - 10.1128/CVI.00105-09 [doi]
AB  - Human leukocyte antigen (HLA) class II genes contribute to the genetic 
      susceptibility to type 1 diabetes (T1D), and susceptible alleles and haplotypes 
      were implicated in the pathogenesis of T1D. This study investigated the 
      heterogeneity in HLA class II haplotype distribution among Tunisian patients with 
      T1D. This was a retrospective case control study done in Monastir in central 
      Tunisia. The subjects comprised 88 T1D patients and 112 healthy controls. 
      HLA-DRB1 and -DQB1 genotyping was done by PCR-sequence-specific priming. 
      Significant DRB1 and DQB1 allelic differences were seen between T1D patients and 
      controls; these differences comprised DRB1*030101 and DQB1*0302, which were 
      higher in T1D patients than in control subjects, and DRB1*070101, DRB1*110101, 
      DQB1*030101, and DQB1*060101, which were lower in T1D patients than in control 
      subjects. In addition, the frequencies of DRB1*030101-DQB1*0201 and 
      DRB1*040101-DQB1*0302 were higher in T1D patients than in control subjects, and 
      the frequencies of DRB1*070101-DQB1*0201 and DRB1*110101-DQB1*030101 haplotypes 
      were lower in T1D patients than in control subjects. Multiple logistic regression 
      analysis revealed the positive association of DRB1*030101-DQB1*0201 and 
      DRB1*040101-DQB1*0302 and the negative association of only DRB1*070101-DQB1*0201 
      haplotypes with T1D. Furthermore, a significantly increased prevalence of 
      DRB1*030101-DQB1*0201 homozygotes was seen for T1D subjects than for control 
      subjects. Our results confirm the association of specific HLA-DR and -DQ alleles 
      and haplotypes with T1D in Tunisians. The identification of similar and unique 
      haplotypes in Tunisians compared to other Caucasians highlights the need for 
      evaluating the contribution of HLA class II to the genetic susceptibility to T1D 
      with regard to haplotype usage and also to ethnic origin and racial background.
FAU - Stayoussef, Mouna
AU  - Stayoussef M
AD  - Research Unit of Hematological and Autoimmune Diseases, Faculty of Pharmacy, 
      University of Monastir, Monastir, Tunisia.
FAU - Benmansour, Jihen
AU  - Benmansour J
FAU - Al-Irhayim, Abdul-Qader
AU  - Al-Irhayim AQ
FAU - Said, Hichem B
AU  - Said HB
FAU - Rayana, Chiheb B
AU  - Rayana CB
FAU - Mahjoub, Touhami
AU  - Mahjoub T
FAU - Almawi, Wassim Y
AU  - Almawi WY
LA  - eng
PT  - Journal Article
DEP - 20090624
PL  - United States
TA  - Clin Vaccine Immunol
JT  - Clinical and vaccine immunology : CVI
JID - 101252125
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DQ beta-Chains)
RN  - 0 (HLA-DQB1 antigen)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (HLA-DRB1 Chains)
RN  - 0 (HLA-DRB1*11:01:01 antigen)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Case-Control Studies
MH  - Child
MH  - Diabetes Mellitus, Type 1/*epidemiology/*genetics/immunology
MH  - Female
MH  - Gene Frequency
MH  - *Genetic Predisposition to Disease
MH  - HLA-DQ Antigens/*genetics
MH  - HLA-DQ beta-Chains
MH  - HLA-DR Antigens/*genetics
MH  - HLA-DRB1 Chains
MH  - Haplotypes
MH  - Humans
MH  - Male
MH  - Polymerase Chain Reaction/methods
MH  - *Polymorphism, Genetic
MH  - Retrospective Studies
MH  - Sequence Analysis, DNA/methods
MH  - Tunisia/epidemiology
MH  - Young Adult
PMC - PMC2725529
EDAT- 2009/06/26 09:00
MHDA- 2009/09/29 06:00
PMCR- 2010/02/01
CRDT- 2009/06/26 09:00
PHST- 2009/06/26 09:00 [entrez]
PHST- 2009/06/26 09:00 [pubmed]
PHST- 2009/09/29 06:00 [medline]
PHST- 2010/02/01 00:00 [pmc-release]
AID - CVI.00105-09 [pii]
AID - 0105-09 [pii]
AID - 10.1128/CVI.00105-09 [doi]
PST - ppublish
SO  - Clin Vaccine Immunol. 2009 Aug;16(8):1146-50. doi: 10.1128/CVI.00105-09. Epub 
      2009 Jun 24.