PMID- 19554729
OWN - NLM
STAT- MEDLINE
DCOM- 20090827
LR  - 20240320
IS  - 0002-7863 (Print)
IS  - 1520-5126 (Electronic)
IS  - 0002-7863 (Linking)
VI  - 130
IP  - 49
DP  - 2008 Dec 10
TI  - Nanoscale growth factor patterns by immobilization on a heparin-mimicking 
      polymer.
PG  - 16585-91
LID - 10.1021/ja803676r [doi]
AB  - In this study, electrostatic interactions between sulfonate groups of an 
      immobilized polymer and the heparin binding domains of growth factors important 
      in cell signaling were exploited to nanopattern the proteins. Poly(sodium 
      4-styrenesulfonate-co-poly(ethylene glycol) methacrylate) (pSS-co-pPEGMA) was 
      synthesized by reversible addition-fragmentation chain transfer (RAFT) 
      polymerization using ethyl S-thiobenzoyl-2-thiopropionate as a chain transfer 
      agent and 2,2'-azoisobutyronitrile (AIBN) as the initiator. The resulting polymer 
      (1) was characterized by 1H NMR, GPC, FT-IR, and UV-vis and had a number average 
      molecular weight (Mn) of 24,000 and a polydispersity index (PDI) of 1.17. The 
      dithioester end group of 1 was reduced to the thiol, and the polymer was 
      subsequently immobilized on a gold substrate. Binding of basic fibroblast growth 
      factor (bFGF) and vascular endothelial growth factor (VEGF) to the polymer via 
      the heparin binding domains was then confirmed by surface plasmon resonance 
      (SPR). The interactions were stable at physiological salt concentrations. Polymer 
      1 was cross-linked onto silicon wafers using an electron beam writer forming 
      micro- and nanopatterns. Resolutions of 100 nm and arbitrary nanoscale features 
      such as concentric circles and contiguous squares and triangles were achieved. 
      Fluorescence microscopy confirmed that bFGF and VEGF were subsequently 
      immobilized to the polymer micro- and nanopatterns.
FAU - Christman, Karen L
AU  - Christman KL
AD  - Department of Chemistry and Biochemistry, University of California, Los Angeles, 
      607 Charles E. Young Drive East, Los Angeles, California 90095-1569, USA.
FAU - Vazquez-Dorbatt, Vimary
AU  - Vazquez-Dorbatt V
FAU - Schopf, Eric
AU  - Schopf E
FAU - Kolodziej, Christopher M
AU  - Kolodziej CM
FAU - Li, Ronald C
AU  - Li RC
FAU - Broyer, Rebecca M
AU  - Broyer RM
FAU - Chen, Yong
AU  - Chen Y
FAU - Maynard, Heather D
AU  - Maynard HD
LA  - eng
GR  - F31 GM077086-02/GM/NIGMS NIH HHS/United States
GR  - R21 EB 005838/EB/NIBIB NIH HHS/United States
GR  - R21 EB005838/EB/NIBIB NIH HHS/United States
GR  - F31 GM077086-04/GM/NIGMS NIH HHS/United States
GR  - R21 EB005838-01/EB/NIBIB NIH HHS/United States
GR  - F31 GM077086/GM/NIGMS NIH HHS/United States
GR  - F32 HL082138-01/HL/NHLBI NIH HHS/United States
GR  - F32 HL082138/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Am Chem Soc
JT  - Journal of the American Chemical Society
JID - 7503056
RN  - 0 (Immobilized Proteins)
RN  - 0 (Methacrylates)
RN  - 0 (Polymers)
RN  - 0 (Sulfonic Acids)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (polyethylene glycol methacrylate)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 9005-49-6 (Heparin)
RN  - ZSL2FB6GXN (styrenesulfonic acid polymer)
SB  - IM
MH  - Biomimetic Materials/chemical synthesis/*chemistry
MH  - Fibroblast Growth Factor 2/*chemistry
MH  - Heparin/*chemistry
MH  - Immobilized Proteins/*chemistry
MH  - Methacrylates/chemistry
MH  - Models, Molecular
MH  - *Nanotechnology
MH  - Polyethylene Glycols/chemistry
MH  - Polymers/chemical synthesis/*chemistry
MH  - Protein Conformation
MH  - Sulfonic Acids/chemistry
MH  - Vascular Endothelial Growth Factor A/*chemistry
PMC - PMC3110987
MID - NIHMS86571
EDAT- 2009/06/26 09:00
MHDA- 2009/08/28 09:00
PMCR- 2011/06/09
CRDT- 2009/06/26 09:00
PHST- 2009/06/26 09:00 [entrez]
PHST- 2009/06/26 09:00 [pubmed]
PHST- 2009/08/28 09:00 [medline]
PHST- 2011/06/09 00:00 [pmc-release]
AID - 10.1021/ja803676r [doi]
PST - ppublish
SO  - J Am Chem Soc. 2008 Dec 10;130(49):16585-91. doi: 10.1021/ja803676r.