PMID- 1955541
OWN - NLM
STAT- MEDLINE
DCOM- 19911230
LR  - 20190510
IS  - 0268-1161 (Print)
IS  - 0268-1161 (Linking)
VI  - 6
IP  - 3
DP  - 1991 Mar
TI  - Concentrations of free oestradiol and progesterone in human preovulatory 
      follicular fluid.
PG  - 359-64
AB  - This study describes the distribution, based on computer calculations, of the 
      total concentration of oestradiol (E2) and progesterone (P4) between a free and a 
      protein-bound fraction in each of 98 preovulatory follicular fluids (FF). The FFs 
      were obtained from 30 women undergoing in-vitro fertilization--embryo transfer 
      (IVF-ET) treatment. The concentrations of free and total steroid were correlated 
      to oocyte cleavage and establishment of pregnancies. In the FF, 4.3% of E2 was 
      free, 1.5% was bound to sex-hormone-binding-globulin (SHBG), 94.2% to albumin and 
      less than 0.1% was bound to cortisol-binding-protein (CBP). The distribution of 
      P4 in FF was 4.1% free, 5.6% bound to CBP, 90.3% bound to albumin and less than 
      0.1% was bound to SHBG. These results demonstrate that albumin plays a central 
      role in maintaining the concentration gradient of steroids between the 
      preovulatory FF and the circulation. The concentration of free E2 in fluid from 
      follicles in which the oocyte cleaved was significantly lower in patients who 
      achieved pregnancy (133 +/- 9 nM) (+/- SEM) than in fluid from follicles in which 
      the oocyte cleaved but where the patient did not become pregnant (169 +/- 13 nM: 
      P less than 0.05). Comparing the same two groups, the total concentration of E2 
      was also significantly lower in FF from patients who became pregnant. By 
      contrast, no such correlation was found for either the free or the total 
      concentrations of P4 in FF.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Andersen, C Y
AU  - Andersen CY
AD  - Department of Obstetrics and Gynaecology, Rigshospitalet, Copenhagen, Denmark.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
RN  - 0 (Albumins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Sex Hormone-Binding Globulin)
RN  - 0 (cortisol binding globulin)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Albumins/metabolism
MH  - Body Fluids/*metabolism
MH  - Carrier Proteins/metabolism
MH  - Cleavage Stage, Ovum/metabolism
MH  - Estradiol/*metabolism
MH  - Female
MH  - Follicular Phase/*physiology
MH  - Humans
MH  - Ovarian Follicle/*metabolism
MH  - Pregnancy/metabolism
MH  - Progesterone/*metabolism
MH  - Sex Hormone-Binding Globulin/metabolism
EDAT- 1991/03/01 00:00
MHDA- 1991/03/01 00:01
CRDT- 1991/03/01 00:00
PHST- 1991/03/01 00:00 [pubmed]
PHST- 1991/03/01 00:01 [medline]
PHST- 1991/03/01 00:00 [entrez]
AID - 10.1093/oxfordjournals.humrep.a137339 [doi]
PST - ppublish
SO  - Hum Reprod. 1991 Mar;6(3):359-64. doi: 10.1093/oxfordjournals.humrep.a137339.