PMID- 19555720
OWN - NLM
STAT- MEDLINE
DCOM- 20091217
LR  - 20220330
IS  - 1879-0542 (Electronic)
IS  - 0165-2478 (Print)
IS  - 0165-2478 (Linking)
VI  - 125
IP  - 2
DP  - 2009 Aug 15
TI  - TLR5 ligation by flagellin converts tolerogenic dendritic cells into activating 
      antigen-presenting cells that preferentially induce T-helper 1 responses.
PG  - 114-8
LID - 10.1016/j.imlet.2009.06.007 [doi]
AB  - Dendritic cells (DCs) differentiated in the presence of IL-10 preferentially 
      induce regulatory T-cells and tolerance. Whether the tolerogenic properties 
      displayed by these DCs (Tol-DCs) can be overcome has not been fully explored. 
      Here we show for the first time that Tol-DCs express higher levels of TLR5 mRNA, 
      but not TLR4 or TLR9 mRNA relative to DCs differentiated with GM-CSF and IL-4 
      (BM-DCs). In response to flagellin, a natural TLR-5 ligand, Tol-DCs produced 
      IL-12 but not IL-10. Unlike Tol-DCs stimulated with LPS, which produce high 
      levels of IL-10 and fail to generate a cognate inflammatory response in CD4(+) 
      T-cells, flagellin-stimulated Tol-DCs promoted the differentiation of CD4(+) T 
      cells with a T-helper 1 phenotype. The divergent T-cell outcomes induced by 
      Tol-DCs in response to different TLR-ligands highlights not only their 
      plasticity, but also points to TLR5 ligation as a potential strategy to overcome 
      tolerance in environments that are otherwise conducive to immune 
      unresponsiveness.
FAU - Vicente-Suarez, Ildelfonso
AU  - Vicente-Suarez I
AD  - Division of Immunology and Division of Malignant Hematology, H. Lee Moffitt 
      Cancer Center & Research Institute, Tampa, FL 33612, United States.
FAU - Brayer, Jason
AU  - Brayer J
FAU - Villagra, Alejandro
AU  - Villagra A
FAU - Cheng, Fengdong
AU  - Cheng F
FAU - Sotomayor, Eduardo M
AU  - Sotomayor EM
LA  - eng
GR  - R01 CA087583/CA/NCI NIH HHS/United States
GR  - CA87583/CA/NCI NIH HHS/United States
GR  - R01 CA100850/CA/NCI NIH HHS/United States
GR  - CA100850/CA/NCI NIH HHS/United States
GR  - R01 CA087583-09/CA/NCI NIH HHS/United States
GR  - R01 CA100850-05/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20090623
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (Receptors, Antigen, T-Cell)
RN  - 0 (Toll-Like Receptor 5)
RN  - 12777-81-0 (Flagellin)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - *Antigen Presentation/drug effects
MH  - Bone Marrow Cells/immunology
MH  - Cell Differentiation/drug effects/immunology
MH  - Dendritic Cells/drug effects/*immunology/metabolism
MH  - Flagellin/pharmacology
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/metabolism
MH  - Interferon-gamma/*metabolism
MH  - Interleukin-10/metabolism
MH  - Interleukin-12/*metabolism
MH  - Interleukin-4/metabolism
MH  - Lymphocyte Activation/drug effects
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Transgenic
MH  - Protein Binding
MH  - Receptors, Antigen, T-Cell
MH  - Th1 Cells/immunology/*metabolism/pathology
MH  - Toll-Like Receptor 5/genetics/immunology/*metabolism
PMC - PMC2758552
MID - NIHMS127332
EDAT- 2009/06/27 09:00
MHDA- 2009/12/18 06:00
PMCR- 2010/08/15
CRDT- 2009/06/27 09:00
PHST- 2009/02/17 00:00 [received]
PHST- 2009/06/12 00:00 [revised]
PHST- 2009/06/15 00:00 [accepted]
PHST- 2009/06/27 09:00 [entrez]
PHST- 2009/06/27 09:00 [pubmed]
PHST- 2009/12/18 06:00 [medline]
PHST- 2010/08/15 00:00 [pmc-release]
AID - S0165-2478(09)00163-1 [pii]
AID - 10.1016/j.imlet.2009.06.007 [doi]
PST - ppublish
SO  - Immunol Lett. 2009 Aug 15;125(2):114-8. doi: 10.1016/j.imlet.2009.06.007. Epub 
      2009 Jun 23.