PMID- 19555760
OWN - NLM
STAT- MEDLINE
DCOM- 20091203
LR  - 20161125
IS  - 1095-953X (Electronic)
IS  - 0969-9961 (Linking)
VI  - 35
IP  - 3
DP  - 2009 Sep
TI  - BDNF regulates BIM expression levels in 3-nitropropionic acid-treated cortical 
      neurons.
PG  - 448-56
LID - 10.1016/j.nbd.2009.06.006 [doi]
AB  - 3-Nitropropionic acid (3-NP) is an irreversible inhibitor of succinate 
      dehydrogenase that has been used to explore the primary mechanisms of cell death 
      associated with mitochondrial dysfunction and neurodegeneration in Huntington's 
      disease. In this study we investigated the ability of brain-derived neurotrophic 
      factor (BDNF) to suppress mitochondrial-dependent cell death induced by 3-NP in 
      primary cortical neurons. This neurotrophin prevented 3-NP-induced release of 
      cytochrome c and Smac/Diablo, caspase-3-like activity and nuclear 
      condensation/fragmentation. Furthermore, it greatly increased phosphorylation of 
      Akt and MAPK, suggesting the involvement of these signalling pathways in BDNF 
      neuroprotection. Interestingly, BDNF decreased the levels of the pro-apoptotic 
      protein Bim in mitochondrial and total cell lysates through the activation of the 
      MEK1/2 pathway. This effect was due to an increase in the degradation rates of 
      Bim. Our data support an important role for BDNF, in protecting cortical neurons 
      against apoptotic cell death caused by inhibition of mitochondrial complex II.
FAU - Almeida, Sandra
AU  - Almeida S
AD  - Institute of Biochemistry, Center for Neuroscience and Cell Biology, University 
      of Coimbra, Coimbra 3004-504, Portugal.
FAU - Laco, Mario
AU  - Laco M
FAU - Cunha-Oliveira, Teresa
AU  - Cunha-Oliveira T
FAU - Oliveira, Catarina R
AU  - Oliveira CR
FAU - Rego, A Cristina
AU  - Rego AC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090623
PL  - United States
TA  - Neurobiol Dis
JT  - Neurobiology of disease
JID - 9500169
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Bcl-2-Like Protein 11)
RN  - 0 (Bcl2l11 protein, rat)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Carrier Proteins)
RN  - 0 (Central Nervous System Agents)
RN  - 0 (Chromatin)
RN  - 0 (DIABLO protein, rat)
RN  - 0 (Membrane Proteins)
RN  - 0 (Mitochondrial Proteins)
RN  - 0 (Nitro Compounds)
RN  - 0 (Propionates)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 9007-43-6 (Cytochromes c)
RN  - EC 1.3.99.1 (Succinate Dehydrogenase)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 3.4.22.- (Casp3 protein, rat)
RN  - EC 3.4.22.- (Caspase 3)
RN  - QY4L0FOX0D (3-nitropropionic acid)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects/*physiology
MH  - Apoptosis Regulatory Proteins/*metabolism
MH  - Bcl-2-Like Protein 11
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Carrier Proteins/metabolism
MH  - Caspase 3/metabolism
MH  - Cells, Cultured
MH  - Central Nervous System Agents/toxicity
MH  - Cerebral Cortex/drug effects/*physiology
MH  - Chromatin/drug effects/metabolism
MH  - Cytochromes c/metabolism
MH  - MAP Kinase Signaling System/physiology
MH  - Membrane Proteins/*metabolism
MH  - Mitochondria/drug effects/physiology
MH  - Mitochondrial Proteins/metabolism
MH  - Neurons/drug effects/*physiology
MH  - Nitro Compounds/toxicity
MH  - Phosphorylation
MH  - Propionates/toxicity
MH  - Proto-Oncogene Proteins/*metabolism
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Succinate Dehydrogenase/antagonists & inhibitors
EDAT- 2009/06/27 09:00
MHDA- 2009/12/16 06:00
CRDT- 2009/06/27 09:00
PHST- 2009/01/15 00:00 [received]
PHST- 2009/06/03 00:00 [revised]
PHST- 2009/06/11 00:00 [accepted]
PHST- 2009/06/27 09:00 [entrez]
PHST- 2009/06/27 09:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
AID - S0969-9961(09)00143-0 [pii]
AID - 10.1016/j.nbd.2009.06.006 [doi]
PST - ppublish
SO  - Neurobiol Dis. 2009 Sep;35(3):448-56. doi: 10.1016/j.nbd.2009.06.006. Epub 2009 
      Jun 23.