PMID- 1955839
OWN - NLM
STAT- MEDLINE
DCOM- 19920102
LR  - 20071115
IS  - 0022-1198 (Print)
IS  - 0022-1198 (Linking)
VI  - 36
IP  - 5
DP  - 1991 Sep
TI  - Report of the blind trial of the Cetus Amplitype HLA DQ alpha forensic 
      deoxyribonucleic acid (DNA) amplification and typing kit.
PG  - 1551-6
AB  - The AmpliType HLA DQ alpha forensic DNA amplification and typing kit is designed 
      for the qualitative analysis of the human leukocyte antigen (HLA) DQ alpha 
      alleles present in deoxyribonucleic acid (DNA) extracted from forensic samples. 
      The AmpliType kit is the first forensic DNA typing product based on the GeneAmp 
      polymerase chain reaction (PCR) process. The kit was evaluated by five forensic 
      science laboratories (test sites) to assess their ability to perform DNA typing 
      using PCR on sample types typically encountered by forensic laboratories. None of 
      the DNA-containing samples was mistyped. Of the 180 DNA-containing samples 
      analyzed, results were reported for 178 (98.9%). Of the 178 samples with results, 
      all were correctly typed. Two sites did not report a result for one sample each. 
      Four of the five laboratories experienced no significant levels of contamination 
      in the DNA-containing samples. At the one site with the highest number of 
      DNA-containing samples with contamination, the typing results were not 
      compromised. This site was able to correct the contamination problem through 
      simple procedural changes and stricter attention to sterile technique. Blank 
      controls were important to monitor contamination. In conclusion, the trial 
      demonstrated that forensic science laboratories are capable of setting up a 
      PCR-based DNA typing laboratory and successfully using the AmpliType HLA DQ alpha 
      forensic DNA amplification and typing kit to analyze forensic samples.
FAU - Walsh, P S
AU  - Walsh PS
AD  - Cetus Corp., Emeryville, CA.
FAU - Fildes, N
AU  - Fildes N
FAU - Louie, A S
AU  - Louie AS
FAU - Higuchi, R
AU  - Higuchi R
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Forensic Sci
JT  - Journal of forensic sciences
JID - 0375370
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (Reagent Kits, Diagnostic)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Alleles
MH  - Blood Stains
MH  - DNA/blood/*chemistry
MH  - Evaluation Studies as Topic
MH  - Female
MH  - Forensic Medicine/*methods
MH  - HLA-DQ Antigens/analysis/*genetics
MH  - Hair/chemistry
MH  - Humans
MH  - Male
MH  - Mouth Mucosa/chemistry
MH  - Polymerase Chain Reaction
MH  - Predictive Value of Tests
MH  - Reagent Kits, Diagnostic
MH  - Semen/chemistry
MH  - Single-Blind Method
MH  - Specimen Handling/standards
MH  - Vagina/chemistry
EDAT- 1991/09/01 00:00
MHDA- 1991/09/01 00:01
CRDT- 1991/09/01 00:00
PHST- 1991/09/01 00:00 [pubmed]
PHST- 1991/09/01 00:01 [medline]
PHST- 1991/09/01 00:00 [entrez]
PST - ppublish
SO  - J Forensic Sci. 1991 Sep;36(5):1551-6.