PMID- 19559725
OWN - NLM
STAT- MEDLINE
DCOM- 20091204
LR  - 20161125
IS  - 1872-678X (Electronic)
IS  - 0165-0270 (Linking)
VI  - 183
IP  - 2
DP  - 2009 Oct 15
TI  - In vivo multimodal imaging of stem cell transplantation in a rodent model of 
      Parkinson's disease.
PG  - 141-8
LID - 10.1016/j.jneumeth.2009.06.022 [doi]
AB  - Stem cell therapy in the nervous system aims to replace the lost neurons and 
      provide functional recovery. However, it is imperative that we understand the in 
      vivo behaviour of these cells post-implantation. We report visualisation of iron 
      oxide labelled bone marrow-derived stem cells (BMSCs) implanted into the striatum 
      of hemi-parkinsonian rats by magnetic resonance imaging (MRI). Functional 
      efficacy of the donor cells was monitored in vivo using the positron emission 
      tomography (PET) radioligand [11C]raclopride. The cells were visible for 28 days 
      by in vivo MRI. BMSCs provided functional recovery demonstrated by a decreased 
      binding of [11C]raclopride. Although, histology confirmed the persistence of 
      donor cells, no tyrosine hydroxylase positive cells were present. This suggests 
      that BMSCs may have a limited paracrine effect and influence functional recovery. 
      We demonstrate, using multimodal imaging, that we can not only track BMSCs but 
      also establish their effects in a pre-clinical model of Parkinson's disease.
FAU - Jackson, Johanna
AU  - Jackson J
AD  - Stem Cell Imaging, MRC Clinical Sciences Centre, Imperial College London, Du Cane 
      Road, London W12 0NN, United Kingdom. johanna.jackson@med.lu.se
FAU - Chapon, Catherine
AU  - Chapon C
FAU - Jones, William
AU  - Jones W
FAU - Hirani, Ella
AU  - Hirani E
FAU - Qassim, Abdullah
AU  - Qassim A
FAU - Bhakoo, Kishore
AU  - Bhakoo K
LA  - eng
GR  - Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090625
PL  - Netherlands
TA  - J Neurosci Methods
JT  - Journal of neuroscience methods
JID - 7905558
RN  - 0 (Antipsychotic Agents)
RN  - 0 (Carbon Isotopes)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Indoles)
RN  - 430K3SOZ7G (Raclopride)
RN  - 47165-04-8 (DAPI)
RN  - 8HW4YBZ748 (Oxidopamine)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
SB  - IM
MH  - Adult Stem Cells/cytology/physiology/transplantation
MH  - Animals
MH  - Antipsychotic Agents/metabolism
MH  - Carbon Isotopes/metabolism
MH  - Diagnostic Imaging/*methods
MH  - *Disease Models, Animal
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Indoles
MH  - Magnetic Resonance Imaging/methods
MH  - Male
MH  - Oxidopamine/toxicity
MH  - Parkinsonian Disorders/chemically induced/*diagnosis/diagnostic imaging/*surgery
MH  - Positron-Emission Tomography/methods
MH  - Raclopride
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Stem Cell Transplantation/*methods
MH  - Time Factors
MH  - Tyrosine 3-Monooxygenase/metabolism
EDAT- 2009/06/30 09:00
MHDA- 2009/12/16 06:00
CRDT- 2009/06/30 09:00
PHST- 2009/05/06 00:00 [received]
PHST- 2009/06/15 00:00 [revised]
PHST- 2009/06/17 00:00 [accepted]
PHST- 2009/06/30 09:00 [entrez]
PHST- 2009/06/30 09:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
AID - S0165-0270(09)00327-6 [pii]
AID - 10.1016/j.jneumeth.2009.06.022 [doi]
PST - ppublish
SO  - J Neurosci Methods. 2009 Oct 15;183(2):141-8. doi: 
      10.1016/j.jneumeth.2009.06.022. Epub 2009 Jun 25.