PMID- 19562212
OWN - NLM
STAT- MEDLINE
DCOM- 20100315
LR  - 20181201
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 134
IP  - 7
DP  - 2009 Jul
TI  - A regenerable immunochip for the rapid determination of 13 different antibiotics 
      in raw milk.
PG  - 1433-9
LID - 10.1039/b817836d [doi]
AB  - Access to high-quality and safe food is a basic need in our community and, 
      consequently, the European Union has defined maximum residue levels (MRLs) for a 
      number of antibacterial compounds. However, despite the obvious demand for 
      quantitative multi-residue detection methods that can be carried out on a routine 
      basis, there is currently a lack in the development of such systems. In 
      particular, an automated multianalyte detection instrument is needed that is 
      capable of quantifying several antibiotics simultaneously within minutes. The 
      newly developed hapten microarrays are designed for the parallel analysis of 13 
      different antibiotics in milk within six minutes by applying an indirect 
      competitive chemiluminescence microarray immunoassay (CL-MIA). To allow multiple 
      analyses, a regenerable microarray chip was developed based on epoxy-activated 
      PEG chip surfaces, onto which microspotted antibiotic derivatives like 
      sulfonamides, beta-lactams, aminoglycosides, fluorquinolones and polyketides are 
      coupled directly without further use of linking agents. Using the chip reader 
      platform MCR 3, this antigen solid phase is stable for at least 50 consecutive 
      analyses.
FAU - Kloth, Katrin
AU  - Kloth K
AD  - Institute of Hydrochemistry and Chair for Analytical Chemistry, Technische 
      Universitat Munchen, Marchioninistrasse 17, D-81377 Munchen, Germany.
FAU - Rye-Johnsen, Maria
AU  - Rye-Johnsen M
FAU - Didier, Andrea
AU  - Didier A
FAU - Dietrich, Richard
AU  - Dietrich R
FAU - Martlbauer, Erwin
AU  - Martlbauer E
FAU - Niessner, Reinhard
AU  - Niessner R
FAU - Seidel, Michael
AU  - Seidel M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090331
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Haptens)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*analysis/immunology
MH  - Equipment Reuse
MH  - Feasibility Studies
MH  - Food Analysis/*instrumentation/*methods
MH  - Haptens/chemistry
MH  - Immunoassay/*instrumentation/*methods
MH  - Microarray Analysis
MH  - Milk/*chemistry
MH  - Polyethylene Glycols/chemistry
MH  - Surface Properties
MH  - Time Factors
EDAT- 2009/06/30 09:00
MHDA- 2010/03/17 06:00
CRDT- 2009/06/30 09:00
PHST- 2009/06/30 09:00 [entrez]
PHST- 2009/06/30 09:00 [pubmed]
PHST- 2010/03/17 06:00 [medline]
AID - 10.1039/b817836d [doi]
PST - ppublish
SO  - Analyst. 2009 Jul;134(7):1433-9. doi: 10.1039/b817836d. Epub 2009 Mar 31.