PMID- 19562747
OWN - NLM
STAT- MEDLINE
DCOM- 20100518
LR  - 20100222
IS  - 1552-4965 (Electronic)
IS  - 1549-3296 (Linking)
VI  - 93
IP  - 1
DP  - 2010 Apr
TI  - In vitro response of monocyte-derived macrophages to a decellularized pericardial 
      biomaterial.
PG  - 280-8
LID - 10.1002/jbm.a.32554 [doi]
AB  - Decellularized tissue-derived heart valves are an example of biomaterials derived 
      from natural scaffolds. These types of implants are increasing in popularity 
      although their in vivo performance is still only poorly understood and has, at 
      times, been catastrophic. It is apparent that better understanding is required 
      before these biomaterials can be used safely. In this study, the human 
      monocyte-derived macrophage (MDM) response to decellularized bovine pericardium 
      (DBP) was used as a model to predict the biological performance of these 
      materials on implantation. Human monocytes differentiated on tissue culture 
      polystyrene (TCPS) for 14 days were trypsinized and reseeded onto DBP, TCPS, and 
      polydimethylsiloxane (PDMS) for 48 h. The MDMs on DBP contained less 
      intracellular and extracellular esterase activity compared with MDMs on TCPS and 
      PDMS, as well as less acid phosphatase activity than on TCPS. As well, 
      morphologically, MDMs on DBP were less spread, less multinucleated and did not 
      display many lamellipodia. Taken together, these data represent the first 
      evidence of the MDM response to intact, native extracellular matrix, 
      demonstrating that these cells reacted with an altered, possibly reduced foreign 
      body response on this natural scaffold compared with the two control surfaces. 
      This in vitro MDM cell model may provide a novel method for predicting and 
      elucidating the biological performance of tissue-derived biomaterials, thereby 
      directing a more rational design of biomaterials for tissue regeneration 
      purposes.
FAU - Ariganello, Marianne B
AU  - Ariganello MB
AD  - Dalhousie University, Halifax, Nova Scotia, Canada. marigane@dal.ca
FAU - Labow, Rosalind S
AU  - Labow RS
FAU - Lee, J Michael
AU  - Lee JM
LA  - eng
GR  - STP53877/Canadian Institutes of Health Research/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 0 (Biocompatible Materials)
RN  - 9007-49-2 (DNA)
RN  - EC 3.1.- (Esterases)
RN  - EC 3.1.3.2 (Acid Phosphatase)
SB  - IM
MH  - Acid Phosphatase/metabolism
MH  - Animals
MH  - Biocompatible Materials/*pharmacology
MH  - Cells, Cultured
MH  - DNA/metabolism
MH  - Esterases/metabolism
MH  - Humans
MH  - Immunoblotting
MH  - Intracellular Space/drug effects/metabolism
MH  - Macrophages/*cytology/*drug effects/enzymology/ultrastructure
MH  - Microscopy, Confocal
MH  - Pericardium/*cytology
EDAT- 2009/06/30 09:00
MHDA- 2010/05/19 06:00
CRDT- 2009/06/30 09:00
PHST- 2009/06/30 09:00 [entrez]
PHST- 2009/06/30 09:00 [pubmed]
PHST- 2010/05/19 06:00 [medline]
AID - 10.1002/jbm.a.32554 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2010 Apr;93(1):280-8. doi: 10.1002/jbm.a.32554.