PMID- 19565906
OWN - NLM
STAT- MEDLINE
DCOM- 20090715
LR  - 20090701
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 542
DP  - 2009
TI  - Immunity of lentiviral vector-modified dendritic cells.
PG  - 245-59
LID - 10.1007/978-1-59745-561-9_13 [doi]
AB  - Innovative approaches to induce a strong immune response are key to the success 
      of immunotherapy. Dendritic cells (DCs) are professional antigen-presenting cells 
      (APCs) equipped with co-stimulatory, adhesion, and major histocompatibility 
      complex (MHC) molecules needed for initiation and reactivation of the immune 
      response. DCs are able to initiate and stimulate both innate and adaptive immune 
      responses and, by secretion of cytokines, chemokines, and expression of 
      regulatory molecules, to shape the adaptive immune response toward a long-lasting 
      memory immunity. DCs from the peripheral blood of immune-compromised patients, 
      however, often display an immature phenotype with defective functions. This 
      emphasizes the importance and potential of engineering antigen-specific DCs in 
      vitro. A state-of-the-art approach to overcome the prevailing immune 
      dysfunction(s) in patients is to engineer DCs or DC progenitors to generate fully 
      functional DCs for the modification of host immunity. Lentiviral vectors (LVs) 
      are highly efficient gene transfer vehicles for engineering DC functions. 
      Examples oflentiviral vectors encoding immune-modulatory genes and useful 
      functional assays for the analysis of effector immune cell response are described 
      in this chapter.
FAU - Han, Shuhong
AU  - Han S
AD  - Department of Molecular Genetics and Microbiology, Powell Gene Therapy Center and 
      McKnight Brain Institute, University of Florida, College of Medicine, 
      Gainesville, FL, USA.
FAU - Chang, Lung-Ji
AU  - Chang LJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Antigens)
RN  - 0 (Epitopes)
RN  - 0 (Immunologic Factors)
SB  - IM
MH  - Antigens/immunology
MH  - CD8-Positive T-Lymphocytes/cytology/immunology
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Cell Separation
MH  - Dendritic Cells/*immunology/*virology
MH  - Epitopes
MH  - Genetic Vectors/*genetics
MH  - Humans
MH  - Immunity/*immunology
MH  - Immunologic Factors/genetics
MH  - Immunophenotyping
MH  - Lentivirus/*genetics
MH  - Lymphocyte Activation
MH  - Lymphocyte Culture Test, Mixed
MH  - Transduction, Genetic
EDAT- 2009/07/02 09:00
MHDA- 2009/07/16 09:00
CRDT- 2009/07/02 09:00
PHST- 2009/07/02 09:00 [entrez]
PHST- 2009/07/02 09:00 [pubmed]
PHST- 2009/07/16 09:00 [medline]
AID - 10.1007/978-1-59745-561-9_13 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2009;542:245-59. doi: 10.1007/978-1-59745-561-9_13.