PMID- 19566654
OWN - NLM
STAT- MEDLINE
DCOM- 20090914
LR  - 20211020
IS  - 1399-3089 (Electronic)
IS  - 0908-665X (Print)
IS  - 0908-665X (Linking)
VI  - 16
IP  - 3
DP  - 2009 May-Jun
TI  - Quantitative tools for assessing the fate of xenotransplanted human 
      stem/progenitor cells in chimeric mice.
PG  - 145-51
LID - 10.1111/j.1399-3089.2009.00526.x [doi]
AB  - BACKGROUND: Identification of transplanted human cells in mouse models is 
      important for studying the biology and therapeutic potential of stem/progenitor 
      cells. As stem/progenitor cells are often transplanted in low numbers, detection 
      of cell engraftment requires sensitive tools. Probes for single copy genes, as 
      well as repetitive genetic elements are available for detecting transplanted 
      cells, although their value relative to one another had not been defined. 
      METHODS: We examined whether human sequences in chimeric mice could be measured 
      with quantitative real-time polymerase chain reactions for Charcot-Marie-Tooth 
      disease, type 1 repeat element, sex-determining region Y, or short tandem repeats 
      (STR) across human leukocyte antigen (HLA) regions, which are distinct from 
      rodent genomes. RESULTS: We found that specific probes for all three candidate 
      approaches successfully identified human cells in mixtures containing human and 
      mouse genomes. However, probes for Charcot-Marie-Tooth disease element or STRs 
      for HLA regions were less effective for low numbers of transplanted human 
      stem/progenitor cells in mice than human sex-determining region on Y-chromosome. 
      None of the approaches could identify transplanted human cells constituting less 
      than one percent of the total cell mass. This required localization of 
      transplanted cells in tissue sections with human-specific in situ hybridization 
      probes. CONCLUSIONS: Quantitative assays with probes for single copy gene 
      sequences, STRs or sex-determining region will be helpful for demonstrating organ 
      repopulation, although initial lower frequency engraftment of human cells in 
      chimeric mice will be most effectively identified by complementary tools, such as 
      in situ localization of human cells in tissues.
FAU - Cheng, Kang
AU  - Cheng K
AD  - Department of Medicine, Marion Bessin Liver Research Center, Albert Einstein 
      College of Medicine, 1300 Morris Park Avenue, Bronx, 10461 NY, USA.
FAU - Gupta, Sanjeev
AU  - Gupta S
LA  - eng
GR  - R01 DK071111/DK/NIDDK NIH HHS/United States
GR  - P30 CA13330/CA/NCI NIH HHS/United States
GR  - R01 DK46952/DK/NIDDK NIH HHS/United States
GR  - R01 DK071111-04/DK/NIDDK NIH HHS/United States
GR  - R01 DK046952/DK/NIDDK NIH HHS/United States
GR  - R01 DK046952-14/DK/NIDDK NIH HHS/United States
GR  - P30 DK041296/DK/NIDDK NIH HHS/United States
GR  - P30 CA013330/CA/NCI NIH HHS/United States
GR  - P30 DK41296/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Xenotransplantation
JT  - Xenotransplantation
JID - 9438793
RN  - 0 (DNA Probes)
RN  - 0 (HLA-A Antigens)
RN  - 0 (Sex-Determining Region Y Protein)
SB  - IM
MH  - Animals
MH  - Charcot-Marie-Tooth Disease/genetics
MH  - DNA Probes/genetics/metabolism
MH  - Female
MH  - Gestational Age
MH  - *Graft Survival
MH  - HLA-A Antigens/genetics
MH  - Humans
MH  - Liver/cytology/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred NOD
MH  - Mice, SCID
MH  - Pancreas/cytology/metabolism
MH  - Pregnancy
MH  - Repetitive Sequences, Nucleic Acid/genetics
MH  - Reproducibility of Results
MH  - Sequence Analysis, DNA
MH  - Sex-Determining Region Y Protein/genetics
MH  - *Stem Cell Transplantation
MH  - *Transplantation Chimera
MH  - *Transplantation, Heterologous
PMC - PMC2787247
MID - NIHMS151093
EDAT- 2009/07/02 09:00
MHDA- 2009/09/15 06:00
PMCR- 2009/12/02
CRDT- 2009/07/02 09:00
PHST- 2009/07/02 09:00 [entrez]
PHST- 2009/07/02 09:00 [pubmed]
PHST- 2009/09/15 06:00 [medline]
PHST- 2009/12/02 00:00 [pmc-release]
AID - XEN526 [pii]
AID - 10.1111/j.1399-3089.2009.00526.x [doi]
PST - ppublish
SO  - Xenotransplantation. 2009 May-Jun;16(3):145-51. doi: 
      10.1111/j.1399-3089.2009.00526.x.