PMID- 19567078
OWN - NLM
STAT- MEDLINE
DCOM- 20091006
LR  - 20090701
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 89
IP  - 8
DP  - 2009 Mar 3
TI  - [Clinical value of aneusomy of chromosomes in exfoliated urothelial cells to 
      predict the recurrence of superficial bladder cancer after complete transurethral 
      resection].
PG  - 548-51
AB  - OBJECTIVE: To analyze the chromosomal karyotype aberrations of exfoliated 
      urothelial cells in superficial bladder cancer patients and the correlation 
      thereof to recurrence of carcinoma. METHODS: Voided urine samples were collected 
      from 42 patients with pathologically confirmed recurrent bladder cancer and 24 
      bladder cancer patients without pathologically confirmed recurrence, all of which 
      had undergone complete transurethral resection and had been followed up for more 
      than 3 years. Fluorescence in situ hybridization (FISH) was used with Spectrum 
      Green to label the chromosome 7 and Spectrum Red to label the chromosome 17 of 
      the exfoliated urothelial cells. RESULTS: The aneuploidy rates of chromosomes 7 
      and 17 were 48.5% (32/66) and 50.0% (33/66) respectively, and the co-aneuploidy 
      rate of chromosomes 7 and 17 was 25.8% (17/66). In the patients with G(2/3) 
      superficial bladder cancer, the aneuploidy rate of chromosomes 17 of those with 
      recurrence was 64.3%, significantly higher than that of patients without 
      recurrence (22.2%, P < 0.05). However, there were not significant differences in 
      the aneuploidy rates of chromosomes 7 and 17 in the pT(a) and pT(1) superficial 
      bladder cancer patients with or without recurrence (all P > 0.05), however, the 
      co-aneuploidy rate of chromosomes 7 and 17 of the patients with recurrence was 
      47.8%, significantly higher than that of the patients without recurrence (12.5%, 
      P < 0.05). Fourteen of the 42 patients with recurrence showed progression, i.e., 
      with increased grade or stage (>/= pT(2)). The aneuploidy rates of chromosomes 7 
      and 17 of these 14 patients were 78.6% and 92.9% respectively, both significantly 
      higher than those of the 28 patients without progression (42.9% and 46.4% 
      respectively, both P < 0.05). CONCLUSION: Abnormality in the chromosomes in 
      exfoliated urothelial cells of superficial bladder cancer patients using FISH 
      technique helps predict recurrence and progression of the cancer.
FAU - Bao, Qing-Bing
AU  - Bao QB
AD  - Department of Urology, Nanjing First Hospital Affiliated to Nanjing Medical 
      University, Nanjing 210006, China.
FAU - Liu, Jun
AU  - Liu J
FAU - Sun, Hong-Bin
AU  - Sun HB
FAU - Su, Jiang-Hao
AU  - Su JH
FAU - Zhu, Jia-Geng
AU  - Zhu JG
FAU - Lin, Jian-Zhong
AU  - Lin JZ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - *Aneuploidy
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 17
MH  - Chromosomes, Human, Pair 7
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Recurrence, Local/diagnosis/*pathology
MH  - Neoplasm Staging
MH  - Postoperative Period
MH  - Urinary Bladder Neoplasms/*pathology
MH  - Urologic Surgical Procedures
MH  - Urothelium/*pathology
EDAT- 2009/07/02 09:00
MHDA- 2009/10/07 06:00
CRDT- 2009/07/02 09:00
PHST- 2009/07/02 09:00 [entrez]
PHST- 2009/07/02 09:00 [pubmed]
PHST- 2009/10/07 06:00 [medline]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2009 Mar 3;89(8):548-51.