PMID- 19567320
OWN - NLM
STAT- MEDLINE
DCOM- 20091005
LR  - 20090701
IS  - 1878-3554 (Electronic)
IS  - 0099-2399 (Linking)
VI  - 35
IP  - 7
DP  - 2009 Jul
TI  - Endodontic and salivary isolates of Enterococcus faecalis integrate into biofilm 
      from human salivary bacteria cultivated in vitro.
PG  - 986-91
LID - 10.1016/j.joen.2009.04.013 [doi]
AB  - INTRODUCTION: The aim of this study was to examine whether Enterococcus faecalis 
      isolates from endodontic patients (from saliva and from a root canal) are able to 
      prevail against salivary bacteria when grown in coculture in a biofilm reactor. 
      METHODS: Saliva that was tested to be free of E. faecalis was used as the 
      inoculum. The fate of E. faecalis was examined by using culture techniques and 
      fluorescence in situ hybridization (FISH). RESULTS: The root canal isolate 
      accounted for 37.4% of the biofilm and 31.9% of the planktonic phase when 
      examined by the culture technique, whereas the proportions examined by FISH 
      showed 15.3% in the biofilm and 11.7% in the planktonic phase. The saliva isolate 
      (as examined by the culture technique) accounted for 32.4% in the biofilm and 
      27.1% in the planktonic phase, respectively, compared with 14.1% in the biofilm 
      and 9.5% in the planktonic phase when examined by FISH analysis. CONCLUSIONS: 
      These results led to the suggestion that E. faecalis could persist in the biofilm 
      of the human oral cavity. Because of the ubiquitous presence of E. faecalis, root 
      canal infections may arise from different sources.
FAU - Al-Ahmad, Ali
AU  - Al-Ahmad A
AD  - Department of Operative Dentistry and Periodontology, Albert-Ludwigs-University, 
      Freiburg, Germany. ali.al-ahmad@uniklinikfreiburg.de
FAU - Muller, Nada
AU  - Muller N
FAU - Wiedmann-Al-Ahmad, Margit
AU  - Wiedmann-Al-Ahmad M
FAU - Sava, Irina
AU  - Sava I
FAU - Hubner, Johannes
AU  - Hubner J
FAU - Follo, Marie
AU  - Follo M
FAU - Schirrmeister, Jorg
AU  - Schirrmeister J
FAU - Hellwig, Elmar
AU  - Hellwig E
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Endod
JT  - Journal of endodontics
JID - 7511484
RN  - 0 (Virulence Factors)
MH  - Animals
MH  - Antibiosis
MH  - Biofilms/*growth & development
MH  - Cattle
MH  - Coculture Techniques
MH  - Colony Count, Microbial
MH  - Dental Enamel/microbiology
MH  - Dental Plaque/microbiology
MH  - Dental Pulp Cavity/*microbiology
MH  - Enterococcus faecalis/*physiology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy, Confocal
MH  - Polymerase Chain Reaction/methods
MH  - Saliva/*microbiology
MH  - Virulence Factors/analysis
EDAT- 2009/07/02 09:00
MHDA- 2009/10/06 06:00
CRDT- 2009/07/02 09:00
PHST- 2009/03/10 00:00 [received]
PHST- 2009/04/06 00:00 [revised]
PHST- 2009/04/08 00:00 [accepted]
PHST- 2009/07/02 09:00 [entrez]
PHST- 2009/07/02 09:00 [pubmed]
PHST- 2009/10/06 06:00 [medline]
AID - S0099-2399(09)00357-4 [pii]
AID - 10.1016/j.joen.2009.04.013 [doi]
PST - ppublish
SO  - J Endod. 2009 Jul;35(7):986-91. doi: 10.1016/j.joen.2009.04.013.