PMID- 19570460
OWN - NLM
STAT- MEDLINE
DCOM- 20120105
LR  - 20090702
IS  - 0529-567X (Print)
IS  - 0529-567X (Linking)
VI  - 44
IP  - 4
DP  - 2009 Apr
TI  - [Genetic analysis of chorionic villi specimen in spontaneous abortion using 
      various methods].
PG  - 253-6
AB  - OBJECTIVE: To explore the efficacy of multiplex ligation-dependent probe 
      amplification (MLPA) combined with fluorescence in situ hybridization (FISH) and 
      comparative genomic hybridization (CGH) combined with FISH in genetic analysis of 
      chorionic villi specimen (CVS) of spontaneous abortion. METHODS: CGH + FISH and 
      MLPA + FISH were used for genetic analysis of 29 CVS from spontaneous abortion 
      and 6 normal CVS from selective abortion, in the mean time, those results were 
      compared with conventional cytogenetic karyotyping. RESULTS: The report time were 
      40 hours in MLPA + FISH and 120 hours in CGH + FISH. The mean time of chorionic 
      villi culture was (240 +/- 72) hours. The successful rate of specimen analysis 
      were 97% (34/35) in CGH, 100% (35/35) in MLPA, 100% (35/35) in FISH and 91% 
      (32/35) in conventional cytogenetic karyotyping. Apart from 1 case failed in CGH 
      analysis, the results from MLPA + FISH were almost similar to that from CGH + 
      FISH, however, that 1 specimen failed in CGH were detected successfully by MLPA + 
      FISH. The discrepancy rate were 13% (4/31) in CGH + FISH and 12% (4/32) in MLPA + 
      FISH respectively when compared with conventional cytogenetic analysis. 
      CONCLUSIONS: MLPA + FISH analysis present shorter detecting time and achieve 100% 
      rate of successful report. This combined method was an important adjuvant 
      approach to conventional cytogenetic karyotyping in CVS from spontaneous 
      abortion.
FAU - Lei, Qiong
AU  - Lei Q
AD  - Reproductive Medicine Center, Department of Obstetrics and Gynecology, First 
      Affiliated Hospital, SUN Yat-sen University, Guangzhou 510080, China.
FAU - Wang, Qiong
AU  - Wang Q
FAU - Zhou, Can-quan
AU  - Zhou CQ
FAU - Chen, Bao-jiang
AU  - Chen BJ
FAU - Chen, Zheng
AU  - Chen Z
FAU - Xu, Yan-wen
AU  - Xu YW
FAU - Zeng, Yan-hong
AU  - Zeng YH
LA  - chi
PT  - Comparative Study
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Fu Chan Ke Za Zhi
JT  - Zhonghua fu chan ke za zhi
JID - 16210370R
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Abortion, Spontaneous/diagnosis/*genetics
MH  - Aneuploidy
MH  - Cells, Cultured
MH  - Chorionic Villi Sampling/*methods
MH  - Chromosome Aberrations
MH  - Comparative Genomic Hybridization/*methods
MH  - Cytogenetic Analysis/methods
MH  - DNA/genetics
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Karyotyping/methods
MH  - Nucleic Acid Amplification Techniques/*methods
MH  - Pregnancy
EDAT- 2009/07/03 09:00
MHDA- 2012/01/06 06:00
CRDT- 2009/07/03 09:00
PHST- 2009/07/03 09:00 [entrez]
PHST- 2009/07/03 09:00 [pubmed]
PHST- 2012/01/06 06:00 [medline]
PST - ppublish
SO  - Zhonghua Fu Chan Ke Za Zhi. 2009 Apr;44(4):253-6.