PMID- 19570955
OWN - NLM
STAT- MEDLINE
DCOM- 20091027
LR  - 20211020
IS  - 1465-2099 (Electronic)
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 90
IP  - Pt 10
DP  - 2009 Oct
TI  - Identification of a novel herpes simplex virus type 1 transcript and protein 
      (AL3) expressed during latency.
PG  - 2342-2352
LID - 10.1099/vir.0.013318-0 [doi]
AB  - The herpes simplex virus type 1 (HSV-1) latency-associated transcript (LAT) is 
      abundantly expressed in latently infected sensory neurons. In small animal models 
      of infection, expression of the first 1.5 kb of LAT coding sequences is necessary 
      and sufficient for wild-type reactivation from latency. The ability of LAT to 
      inhibit apoptosis is important for reactivation from latency. Within the first 
      1.5 kb of LAT coding sequences and LAT promoter sequences, additional transcripts 
      have been identified. For example, the anti-sense to LAT transcript (AL) is 
      expressed in the opposite direction to LAT from the 5' end of LAT and LAT 
      promoter sequences. In addition, the upstream of LAT (UOL) transcript is 
      expressed in the LAT direction from sequences in the LAT promoter. Further 
      examination of the first 1.5 kb of LAT coding sequences revealed two small ORFs 
      that are anti-sense with respect to LAT (AL2 and AL3). A transcript spanning AL3 
      was detected in productively infected cells, mouse neuroblastoma cells stably 
      expressing LAT and trigeminal ganglia (TG) of latently infected mice. 
      Peptide-specific IgG directed against AL3 specifically recognized a protein 
      migrating near 15 kDa in cells stably transfected with LAT, mouse neuroblastoma 
      cells transfected with a plasmid containing the AL3 ORF and TG of latently 
      infected mice. The inability to detect the AL3 protein during productive 
      infection may have been because the 5' terminus of the AL3 transcript was 
      downstream of the first in-frame methionine of the AL3 ORF during productive 
      infection.
FAU - Jaber, Tareq
AU  - Jaber T
AD  - School of Biological Sciences, Nebraska Center for Virology, University of 
      Nebraska, Lincoln, NE 68503, USA.
FAU - Henderson, Gail
AU  - Henderson G
AD  - Department of Veterinary and Biomedical Sciences, Nebraska Center for Virology, 
      University of Nebraska, Lincoln, NE 68503, USA.
FAU - Li, Sumin
AU  - Li S
AD  - Department of Veterinary and Biomedical Sciences, Nebraska Center for Virology, 
      University of Nebraska, Lincoln, NE 68503, USA.
FAU - Perng, Guey-Chuen
AU  - Perng GC
AD  - Department of Pathology and Laboratory Medicine, Emory Vaccine Center, Emory 
      University School of Medicine, Atlanta, GA 30322, USA.
FAU - Carpenter, Dale
AU  - Carpenter D
AD  - The Gavin Herbert Eye Institute, School of Medicine, University of California 
      Irvine, Irvine, CA 92697-4375, USA.
FAU - Wechsler, Steven L
AU  - Wechsler SL
AD  - Center for Virus Research, University of California Irvine, Irvine, CA 92697, 
      USA.
AD  - Department of Microbiology and Molecular Genetics, School of Medicine, University 
      of California Irvine, Irvine, CA 92697, USA.
AD  - The Gavin Herbert Eye Institute, School of Medicine, University of California 
      Irvine, Irvine, CA 92697-4375, USA.
FAU - Jones, Clinton
AU  - Jones C
AD  - Department of Veterinary and Biomedical Sciences, Nebraska Center for Virology, 
      University of Nebraska, Lincoln, NE 68503, USA.
AD  - School of Biological Sciences, Nebraska Center for Virology, University of 
      Nebraska, Lincoln, NE 68503, USA.
LA  - eng
GR  - 1P20RR15635/RR/NCRR NIH HHS/United States
GR  - R01 EY013191/EY/NEI NIH HHS/United States
GR  - R21AI069176/AI/NIAID NIH HHS/United States
GR  - R21 AI069176/AI/NIAID NIH HHS/United States
GR  - EY13191/EY/NEI NIH HHS/United States
GR  - R01 NS049556/NS/NINDS NIH HHS/United States
GR  - P20 RR015635/RR/NCRR NIH HHS/United States
GR  - NS049556/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20090701
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cell Line, Tumor
MH  - Female
MH  - Gene Expression Regulation, Viral/*physiology
MH  - Herpesvirus 1, Human/*physiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Trigeminal Ganglion/virology
MH  - Viral Proteins/genetics/*metabolism
MH  - Virus Latency/*physiology
PMC - PMC2888094
EDAT- 2009/07/03 09:00
MHDA- 2009/10/29 06:00
PMCR- 2010/10/01
CRDT- 2009/07/03 09:00
PHST- 2009/07/03 09:00 [entrez]
PHST- 2009/07/03 09:00 [pubmed]
PHST- 2009/10/29 06:00 [medline]
PHST- 2010/10/01 00:00 [pmc-release]
AID - 2342 [pii]
AID - 10.1099/vir.0.013318-0 [doi]
PST - ppublish
SO  - J Gen Virol. 2009 Oct;90(Pt 10):2342-2352. doi: 10.1099/vir.0.013318-0. Epub 2009 
      Jul 1.