PMID- 19571023
OWN - NLM
STAT- MEDLINE
DCOM- 20091026
LR  - 20211020
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Print)
IS  - 0095-1137 (Linking)
VI  - 47
IP  - 9
DP  - 2009 Sep
TI  - A trilocus sequence typing scheme for hospital epidemiology and subspecies 
      differentiation of an important nosocomial pathogen, Enterococcus faecalis.
PG  - 2713-9
LID - 10.1128/JCM.00667-09 [doi]
AB  - In this study, we present a trilocus sequence typing (TLST) scheme based on 
      intragenic regions of two antigenic genes, ace and salA (encoding a 
      collagen/laminin adhesin and a cell wall-associated antigen, respectively), and a 
      gene associated with antibiotic resistance, lsa (encoding a putative ABC 
      transporter), for subspecies differentiation of Enterococcus faecalis. Each of 
      the alleles was analyzed using 50 E. faecalis isolates representing 42 diverse 
      multilocus sequence types (ST(M); based on seven housekeeping genes) and four 
      groups of clonally linked (by pulsed-field gel electrophoresis [PFGE]) isolates. 
      The allelic profiles and/or concatenated sequences of the three genes agreed with 
      multilocus sequence typing (MLST) results for typing of 49 of the 50 isolates; in 
      addition to the one exception, two isolates were found to have identical TLST 
      types but were single-locus variants (differing by a single nucleotide) by MLST 
      and were therefore also classified as clonally related by MLST. TLST was also 
      comparable to PFGE for establishing short-term epidemiological relationships, 
      typing all isolates classified as clonally related by PFGE with the same type. 
      TLST was then applied to representative isolates (of each PFGE subtype and 
      isolation year) of a collection of 48 hospital isolates and demonstrated the same 
      relationships between isolates of an outbreak strain as those found by MLST and 
      PFGE. In conclusion, the TLST scheme described here was shown to be successful 
      for investigating short-term epidemiology in a hospital setting and may provide 
      an alternative to MLST for discriminating isolates.
FAU - Chowdhury, Shahreen A
AU  - Chowdhury SA
AD  - Division of Infectious Diseases, Department of Internal Medicine, University of 
      Texas Medical School at Houston, 6431 Fannin Street, Houston, TX 77030, USA.
FAU - Arias, Cesar A
AU  - Arias CA
FAU - Nallapareddy, Sreedhar R
AU  - Nallapareddy SR
FAU - Reyes, Jinnethe
AU  - Reyes J
FAU - Willems, Rob J L
AU  - Willems RJ
FAU - Murray, Barbara E
AU  - Murray BE
LA  - eng
GR  - R37 AI 47923/AI/NIAID NIH HHS/United States
GR  - K99 AI072961/AI/NIAID NIH HHS/United States
GR  - 1K99-AI72961/AI/NIAID NIH HHS/United States
GR  - UL1 RR024148/RR/NCRR NIH HHS/United States
GR  - R37 AI047923/AI/NIAID NIH HHS/United States
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20090701
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Ace protein, Enterococcus)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Lsa protein, Enterococcus faecalis)
SB  - IM
MH  - ATP-Binding Cassette Transporters/genetics
MH  - Animals
MH  - Bacterial Proteins/genetics
MH  - Bacterial Typing Techniques/*methods
MH  - Carrier Proteins/genetics
MH  - Cross Infection/*microbiology
MH  - DNA Fingerprinting/*methods
MH  - DNA, Bacterial/chemistry/genetics
MH  - Disease Outbreaks
MH  - Enterococcus faecalis/*classification/genetics/*isolation & purification
MH  - Genotype
MH  - Gram-Positive Bacterial Infections/*diagnosis/*microbiology
MH  - Humans
MH  - Sequence Analysis, DNA/*methods
PMC - PMC2738112
EDAT- 2009/07/03 09:00
MHDA- 2009/10/27 06:00
PMCR- 2010/03/01
CRDT- 2009/07/03 09:00
PHST- 2009/07/03 09:00 [entrez]
PHST- 2009/07/03 09:00 [pubmed]
PHST- 2009/10/27 06:00 [medline]
PHST- 2010/03/01 00:00 [pmc-release]
AID - JCM.00667-09 [pii]
AID - 0667-09 [pii]
AID - 10.1128/JCM.00667-09 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2009 Sep;47(9):2713-9. doi: 10.1128/JCM.00667-09. Epub 2009 Jul 
      1.