PMID- 19575438
OWN - NLM
STAT- MEDLINE
DCOM- 20091216
LR  - 20091026
IS  - 1097-0290 (Electronic)
IS  - 0006-3592 (Linking)
VI  - 104
IP  - 5
DP  - 2009 Dec 1
TI  - Bacterial artificial chromosome library for genome-wide analysis of Chinese 
      hamster ovary cells.
PG  - 986-94
LID - 10.1002/bit.22463 [doi]
AB  - Chinese hamster ovary (CHO) cell lines are widely used for scientific research 
      and biotechnology. A CHO genomic bacterial artificial chromosome (BAC) library 
      was constructed from a mouse dihydrofolate reductase (DHFR) gene-amplified CHO 
      DR1000L-4N cell line for genome-wide analysis of CHO cell lines. The CHO BAC 
      library consisted of 122,281 clones and was expected to cover the entire CHO 
      genome five times. A CHO chromosomal map was constructed by fluorescence in situ 
      hybridization (FISH) imaging using BAC clones as hybridization probes (BAC-FISH). 
      Thirteen BAC-FISH marker clones were necessary to identify all the 20 individual 
      chromosomes in a DHFR-deficient CHO DG44 cell line because of the aneuploidy of 
      the cell line. To determine the genomic structure of the exogenous Dhfr amplicon, 
      a 165-kb DNA region containing exogenous Dhfr was cloned from the BAC library 
      using high-density replica (HDR) filters and Southern blot analysis. The 
      nucleotide sequence analysis revealed a novel genomic structure in which the 
      vector sequence containing Dhfr was sandwiched by long inverted sequences of the 
      CHO genome.
FAU - Omasa, Takeshi
AU  - Omasa T
AD  - Department of Biotechnology, Graduate School of Engineering, Osaka University, 
      2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. omasa@bio.eng.osaka-u.ac.jp
FAU - Cao, Yihua
AU  - Cao Y
FAU - Park, Joon Young
AU  - Park JY
FAU - Takagi, Yasuhiro
AU  - Takagi Y
FAU - Kimura, Shuichi
AU  - Kimura S
FAU - Yano, Hidenori
AU  - Yano H
FAU - Honda, Kohsuke
AU  - Honda K
FAU - Asakawa, Shuichi
AU  - Asakawa S
FAU - Shimizu, Nobuyoshi
AU  - Shimizu N
FAU - Ohtake, Hisao
AU  - Ohtake H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biotechnol Bioeng
JT  - Biotechnology and bioengineering
JID - 7502021
RN  - 0 (Recombinant Proteins)
RN  - EC 1.5.1.3 (Tetrahydrofolate Dehydrogenase)
SB  - IM
MH  - Animals
MH  - *CHO Cells
MH  - Chromosome Mapping
MH  - *Chromosomes, Artificial, Bacterial
MH  - Cricetinae
MH  - Cricetulus
MH  - *Gene Library
MH  - Genetic Vectors
MH  - *Genome
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Recombinant Proteins/genetics
MH  - Tetrahydrofolate Dehydrogenase/genetics
EDAT- 2009/07/04 09:00
MHDA- 2009/12/17 06:00
CRDT- 2009/07/04 09:00
PHST- 2009/07/04 09:00 [entrez]
PHST- 2009/07/04 09:00 [pubmed]
PHST- 2009/12/17 06:00 [medline]
AID - 10.1002/bit.22463 [doi]
PST - ppublish
SO  - Biotechnol Bioeng. 2009 Dec 1;104(5):986-94. doi: 10.1002/bit.22463.