PMID- 19575963
OWN - NLM
STAT- MEDLINE
DCOM- 20101214
LR  - 20090706
IS  - 0412-4081 (Print)
IS  - 0412-4081 (Linking)
VI  - 45
IP  - 4
DP  - 2009 Apr
TI  - [Relationship between vasculogenic mimicry and clinical pathological characters 
      in retinoblastoma].
PG  - 318-22
AB  - OBJECTIVE: To explore if vasculogenic mimicry (VM) exists in retinoblastoma (Rb) 
      and to explore the clinical significance of VM. METHODS: It was an experimental 
      study. Sixty Rb specimens with complete clinical and prognostic data were 
      collected. Periodic acid-Schif staining and immunohistochemical staining of CD34 
      were conducted to explore if VM exists in those Rb specimens. The expression of 
      HIF-1alpha and VEGF were examined by immunohistochemical staining. The expression 
      of CD34 endothelial antigen was used to label the neo-microvessels. Microvessel 
      density (MVD) in retinoblastoma tissues was calculated. RESULTS: In HE staining 
      slides, VM was present in Rb specimens and was formed by tumor cells but not 
      endothelial cells. Red blood cells were present in the VM. VM existed in 18.33% 
      (11/60) of the Rb specimens. Low R-E graded Rb specimens exhibited a higher VM 
      positive rate than that in the high R-E graded Rb (chi(2) = 8.861, P < 0.05). The 
      positive rate of VM was 4.34% in differentiated type of Rb and was 22.02% in 
      undifferentiated type of Rb (chi(2) = 4.872, P < 0.05). HIF-1alpha and VEGF 
      expressions in Rb with VM were significantly greater than those in Rb without VM 
      (P = 0.001). The density of endothelial vessels correlated with VM. The mean MVD 
      was 49.77 +/- 2.05 in Rb without VM and 36.53 +/- 1.15 in Rb with VM (P < 0.05). 
      CONCLUSIONS: VM exists in Rb. Highly differentiated Rb exhibits more VM than that 
      in less differentiated Rb. Expression of HIF-1alpha and VEGF is greater in Rb 
      with VM, indicating that these factors may stimulate the occurrence of VM.
FAU - Niu, Ying-jun
AU  - Niu YJ
AD  - Department of Ophthalmology, Affiliated Hospital of Qingdao University Medical 
      College, Qingdao 266003, China. niuyingjun@yahoo.com.cn
FAU - Liu, Fu-ling
AU  - Liu FL
FAU - Yang, Ying
AU  - Yang Y
FAU - Yuan, Chun-yan
AU  - Yuan CY
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yan Ke Za Zhi
JT  - [Zhonghua yan ke za zhi] Chinese journal of ophthalmology
JID - 16210540R
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (VEGFA protein, human)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Humans
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/metabolism
MH  - Infant
MH  - Male
MH  - Neovascularization, Pathologic
MH  - Prognosis
MH  - Retinal Neoplasms/*blood supply/*pathology
MH  - Retinoblastoma/*blood supply/*pathology
MH  - Vascular Endothelial Growth Factor A/metabolism
EDAT- 2009/07/07 09:00
MHDA- 2010/12/16 06:00
CRDT- 2009/07/07 09:00
PHST- 2009/07/07 09:00 [entrez]
PHST- 2009/07/07 09:00 [pubmed]
PHST- 2010/12/16 06:00 [medline]
PST - ppublish
SO  - Zhonghua Yan Ke Za Zhi. 2009 Apr;45(4):318-22.