PMID- 19576389
OWN - NLM
STAT- MEDLINE
DCOM- 20090908
LR  - 20131121
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 647
IP  - 1
DP  - 2009 Aug 4
TI  - Portable microfluidic system for determination of urinary creatinine.
PG  - 78-83
LID - 10.1016/j.aca.2009.05.014 [doi]
AB  - A simple, low cost and portable microfluidic system based on a two-point alkaline 
      picrate kinetic reaction has been developed for the determination of urinary 
      creatinine. The creatinine reacts with picric acid under alkaline conditions, 
      forming an orange-red colour, which is monitored on PDMS microchip using a 
      portable miniature fibre optic spectrometer at 510 nm. A linear range was 
      displayed from 0 to 40 mg L(-1) creatinine (r2 = 0.997) with a detection limit of 
      3.3 mg L(-1) (S/N = 3). On-chip absorbance signals are reproducible, with 
      relative standard deviations (RSDs) of 7.1%, when evaluated with 20 mg L(-1) 
      creatinine (n = 10). The standard curves in which the intra-run CVs (4.7-6.8%) 
      and inter-run CVs (7.9%) obtained were performed on three different days and 
      exhibited good reproducibility. The method was highly correlated with the 
      conventional spectrophotometric method when real urine samples were evaluated (r2 
      = 0.948; n = 15).
FAU - Songjaroen, Temsiri
AU  - Songjaroen T
AD  - Graduate Program in Clinical Biochemistry and Molecular Medicine, Faculty of 
      Allied Health Sciences, Chulalongkorn University, Bangkok, 10330, Thailand.
FAU - Maturos, Thitima
AU  - Maturos T
FAU - Sappat, Aussawapong
AU  - Sappat A
FAU - Tuantranont, Adisorn
AU  - Tuantranont A
FAU - Laiwattanapaisal, Wanida
AU  - Laiwattanapaisal W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090520
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (Picrates)
RN  - A49OS0F91S (picric acid)
RN  - AYI8EX34EU (Creatinine)
SB  - IM
MH  - Creatinine/*urine
MH  - Hydrogen-Ion Concentration
MH  - Microfluidics/*instrumentation/*methods
MH  - Picrates/chemistry
MH  - Reproducibility of Results
MH  - Temperature
MH  - Time Factors
EDAT- 2009/07/07 09:00
MHDA- 2009/09/09 06:00
CRDT- 2009/07/07 09:00
PHST- 2009/03/04 00:00 [received]
PHST- 2009/05/05 00:00 [revised]
PHST- 2009/05/12 00:00 [accepted]
PHST- 2009/07/07 09:00 [entrez]
PHST- 2009/07/07 09:00 [pubmed]
PHST- 2009/09/09 06:00 [medline]
AID - S0003-2670(09)00671-0 [pii]
AID - 10.1016/j.aca.2009.05.014 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2009 Aug 4;647(1):78-83. doi: 10.1016/j.aca.2009.05.014. Epub 
      2009 May 20.