PMID- 19576438
OWN - NLM
STAT- MEDLINE
DCOM- 20090914
LR  - 20131121
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 79
IP  - 3
DP  - 2009 Aug 15
TI  - Speciation of selenomethionine and selenocystine using online micro-column 
      containing Cu(II) loaded nanometer-sized Al2O3 coupled with ICP-MS detection.
PG  - 734-8
LID - 10.1016/j.talanta.2009.04.063 [doi]
AB  - A flow injection online speciation procedure by using micro-column packed with 
      Cu(II) loaded nanometer-sized Al(2)O(3) coupled to inductively coupled plasma 
      mass spectrometry (ICP-MS) for the separation and determination of 
      selenomethionine (SeMet) and selenocystine (SeCys(2)) has been developed. The 
      main factors affecting the separation and preconcentration of SeMet and SeCys(2) 
      including pH value, sample flow rate, eluent concentration, eluent volume and 
      flow rate, and interfering ions have been investigated. It was found that 
      SeCys(2) could be selectively retained by micro-column packed with Cu(II) loaded 
      nanometer-sized Al(2)O(3) at pH 4.0, and the retained SeCys(2) could be eluted by 
      1.0 mol L(-1) HNO(3), while SeMet was not retained and passed through the 
      micro-column directly at this pH. Both SeMet and SeCys(2) could be quantitatively 
      adsorbed by the micro-column at pH 9.0, and the retained SeMet and SeCys(2) could 
      be easily eluted with 1.0 mol L(-1) HNO(3). The content of SeMet was obtained by 
      subtracting the SeCys(2) from the total content of seleno amino acids. With the 
      enrichment factor of 7.8 and 7.7, the limits of detection (LODs) for SeMet and 
      SeCys(2) were found to be 24 pg Se mL(-1) and 21 pg Se mL(-1), respectively. The 
      relative standard deviations (RSDs) for SeCys(2) and SeMet with seven replicate 
      determinations of 1.0 ng mL(-1) SeMet and SeCys(2), were 2.1% and 1.6%, 
      respectively, the sampling frequency of 8h(-1) was obtained. The proposed method 
      was applied to the speciation of SeMet and SeCys(2) in selenized yeast, human 
      urine and serum with satisfactory results.
FAU - Duan, Jiankun
AU  - Duan J
AD  - Department of Chemistry, Wuhan University, Wuhan 430072, China.
FAU - Hu, Bin
AU  - Hu B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090507
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0CH9049VIS (Selenocysteine)
RN  - 789U1901C5 (Copper)
RN  - 964MRK2PEL (Selenomethionine)
RN  - LMI26O6933 (Aluminum Oxide)
SB  - IM
MH  - Adsorption
MH  - Aluminum Oxide/*chemistry
MH  - Analytic Sample Preparation Methods
MH  - Copper/*chemistry
MH  - Humans
MH  - Mass Spectrometry
MH  - Nanoparticles/*chemistry
MH  - Selenocysteine/*analysis/blood/chemistry/urine
MH  - Selenomethionine/*analysis/blood/chemistry/urine
MH  - Yeasts/chemistry
EDAT- 2009/07/07 09:00
MHDA- 2009/09/15 06:00
CRDT- 2009/07/07 09:00
PHST- 2008/12/15 00:00 [received]
PHST- 2009/04/24 00:00 [revised]
PHST- 2009/04/29 00:00 [accepted]
PHST- 2009/07/07 09:00 [entrez]
PHST- 2009/07/07 09:00 [pubmed]
PHST- 2009/09/15 06:00 [medline]
AID - S0039-9140(09)00368-3 [pii]
AID - 10.1016/j.talanta.2009.04.063 [doi]
PST - ppublish
SO  - Talanta. 2009 Aug 15;79(3):734-8. doi: 10.1016/j.talanta.2009.04.063. Epub 2009 
      May 7.