PMID- 19576462
OWN - NLM
STAT- MEDLINE
DCOM- 20090914
LR  - 20131121
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 79
IP  - 3
DP  - 2009 Aug 15
TI  - Determination of low level nitrite and nitrate in biological, food and 
      environmental samples by gas chromatography-mass spectrometry and liquid 
      chromatography with fluorescence detection.
PG  - 900-4
LID - 10.1016/j.talanta.2009.05.016 [doi]
AB  - Gas chromatography-mass spectrometry (GC-MS) and liquid chromatography with 
      fluorescence detection (LC-FL) methods have been proposed for the determination 
      of low level nitrite and nitrate in biological, food and environmental samples. 
      The methods include derivatization of aqueous nitrite with 2,3-diaminonaphthalene 
      (DAN), enzymatic reduction of nitrate to nitrite, extraction with toluene and 
      chromatographic analyses of highly fluorescent 2,3-naphthotriazole (NAT) 
      derivative of nitrite by using GC-MS in selected-ion-monitoring (SIM) mode and 
      LC-FL. Nitrite and nitrate ions in solid samples were extracted with 0.5M aqueous 
      NaOH by sonication. The recoveries of nitrite and nitrate ions based on GC-MS and 
      LC-FL results were 98.40% and 98.10% and the precision of these methods, as 
      indicated by the relative standard deviations (RSDs) were 1.00% for nitrite and 
      1.20% for nitrate, respectively. The limits of detection of the GC-MS in SIM mode 
      and LC-FL methods based on S/N=3 were 0.02 and 0.29 pg/ml for nitrite and 0.03 
      and 0.30 pg/ml for nitrate, respectively.
FAU - Akyuz, Mehmet
AU  - Akyuz M
AD  - Zonguldak Karaelmas University, Faculty of Arts and Sciences, Department of 
      Chemistry, 67100 Zonguldak, Turkey. makyuz2004@yahoo.co.uk
FAU - Ata, Sevket
AU  - Ata S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090522
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Nitrates)
RN  - 0 (Nitrites)
RN  - 0 (Triazoles)
RN  - 0 (Water Pollutants, Chemical)
RN  - 059QF0KO0R (Water)
RN  - 269-12-5 (1H-naphtho(2,3-d)triazole)
RN  - 2BNZ6BRS87 (2,3-diaminonaphthalene)
RN  - 53-59-8 (NADP)
RN  - 55X04QC32I (Sodium Hydroxide)
RN  - CKR7XL41N4 (2-Naphthylamine)
RN  - EC 1.7.99.4 (Nitrate Reductase)
SB  - IM
MH  - 2-Naphthylamine/analogs & derivatives/chemistry
MH  - Chromatography, Gas
MH  - Chromatography, Liquid
MH  - *Environment
MH  - Food Analysis/*methods
MH  - Gastric Juice/chemistry
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Mass Spectrometry
MH  - NADP/chemistry/metabolism
MH  - Nitrate Reductase/chemistry/metabolism
MH  - Nitrates/*analysis/blood/chemistry/urine
MH  - Nitrites/*analysis/blood/chemistry/urine
MH  - Reproducibility of Results
MH  - Sodium Hydroxide/chemistry
MH  - Sonication
MH  - Spectrometry, Fluorescence
MH  - Triazoles/chemistry
MH  - Water/chemistry
MH  - Water Pollutants, Chemical/*analysis/blood/chemistry/urine
EDAT- 2009/07/07 09:00
MHDA- 2009/09/15 06:00
CRDT- 2009/07/07 09:00
PHST- 2008/11/18 00:00 [received]
PHST- 2009/04/30 00:00 [revised]
PHST- 2009/05/13 00:00 [accepted]
PHST- 2009/07/07 09:00 [entrez]
PHST- 2009/07/07 09:00 [pubmed]
PHST- 2009/09/15 06:00 [medline]
AID - S0039-9140(09)00397-X [pii]
AID - 10.1016/j.talanta.2009.05.016 [doi]
PST - ppublish
SO  - Talanta. 2009 Aug 15;79(3):900-4. doi: 10.1016/j.talanta.2009.05.016. Epub 2009 
      May 22.