PMID- 19577292
OWN - NLM
STAT- MEDLINE
DCOM- 20091012
LR  - 20090804
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Linking)
VI  - 30
IP  - 28
DP  - 2009 Oct
TI  - Osteogenic and adipogenic differentiation of rat bone marrow cells on 
      non-mulberry and mulberry silk gland fibroin 3D scaffolds.
PG  - 5019-30
LID - 10.1016/j.biomaterials.2009.05.064 [doi]
AB  - This study investigates the potential of 3D silk scaffolds fabricated using 
      tropical tasar non-mulberry, Antheraea mylitta and mulberry, Bombyx mori silk 
      gland fibroin proteins as substrate for osteogenic and adipogenic differentiation 
      of rat bone marrow cells (BMCs). The scaffolds are mechanically robust and show 
      homogenous pore distribution with high porosity and interconnected pore walls. 
      Low immunogenicity of fabricated silk scaffolds as estimated through TNF alpha 
      release indicates its potential as future biopolymeric graft material. Rat bone 
      marrow cells cultured on scaffolds for 28 days under static conditions in 
      osteogenic and adipogenic media respectively led to induction of differentiation. 
      Proliferation and spreading of fibroblasts and bone marrow cells on silk 
      scaffolds were observed to be dependent on scaffold porosity as revealed through 
      confocal microscopic observations. Histological analysis shows osteogenic 
      differentiation within silk scaffolds resulting in extensive mineralization in 
      the form of deposited nodules as observed through intense Alizarin Red S 
      staining. Similarly, adipogenesis was marked by the presence of lipid droplets 
      within scaffolds on staining with Oil Red O. Real-time PCR studies reveal higher 
      transcript levels for osteopontin (Spp1), osteocalcin (Bglap2) and osteonectin 
      (Sparc) genes under osteogenic conditions. Similarly, upregulated adipogenic gene 
      expression was observed within A. mylitta and B. mori scaffolds under adipogenic 
      conditions for Peroxisome proliferator activated receptor gamma (PPARgamma2), 
      lipoprotein lipase (LPL) and adipocyte binding protein (aP2) genes. The results 
      suggest suitability of silk fibroin protein 3D scaffolds as natural biopolymer 
      for potential bone and adipose tissue engineering applications.
FAU - Mandal, Biman B
AU  - Mandal BB
AD  - Department of Biotechnology, Indian Institute of Technology, Kharagpur 721 302, 
      West Bengal, India.
FAU - Kundu, Subhas C
AU  - Kundu SC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090703
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Biocompatible Materials)
RN  - 9007-76-5 (Fibroins)
SB  - IM
MH  - *Adipogenesis
MH  - Animals
MH  - Biocompatible Materials/chemistry
MH  - Bombyx/chemistry
MH  - Bone Marrow Cells/*cytology/metabolism
MH  - Cell Adhesion
MH  - Cell Line
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Fibroins/*chemistry/isolation & purification
MH  - Gene Expression
MH  - Macrophages/cytology
MH  - Male
MH  - Moths/chemistry
MH  - *Osteogenesis
MH  - Rats
MH  - Rats, Inbred BB
MH  - Tissue Engineering/methods
MH  - Tissue Scaffolds/*chemistry
EDAT- 2009/07/07 09:00
MHDA- 2009/10/13 06:00
CRDT- 2009/07/07 09:00
PHST- 2009/03/30 00:00 [received]
PHST- 2009/05/25 00:00 [accepted]
PHST- 2009/07/07 09:00 [entrez]
PHST- 2009/07/07 09:00 [pubmed]
PHST- 2009/10/13 06:00 [medline]
AID - S0142-9612(09)00593-6 [pii]
AID - 10.1016/j.biomaterials.2009.05.064 [doi]
PST - ppublish
SO  - Biomaterials. 2009 Oct;30(28):5019-30. doi: 10.1016/j.biomaterials.2009.05.064. 
      Epub 2009 Jul 3.