PMID- 19580783
OWN - NLM
STAT- MEDLINE
DCOM- 20090820
LR  - 20201209
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 387
IP  - 2
DP  - 2009 Sep 18
TI  - Cellular protein TTRAP interacts with HIV-1 integrase to facilitate viral 
      integration.
PG  - 256-60
LID - 10.1016/j.bbrc.2009.06.153 [doi]
AB  - TTRAP is a PML-NB protein that is involved in the NF-kappaB signaling pathway. 
      TTRAP was recently identified by yeast two-hybrid analysis as a HIV-1 integrase 
      (HIV-1 IN) interacting protein. This interaction was verified by 
      co-immunoprecipitation, GST pull-down, and intracellular imaging, and deletion 
      assays suggested that the N-terminal 180 residues of TTRAP are responsible for 
      the interaction. In stable TTRAP knock-down cell lines, the integration of viral 
      vectors decreased significantly compared with non-silenced cell lines. 
      Conversely, overexpression of TTRAP by transient transfection increased the 
      percentage of integration events. This is the first time that TTRAP has been 
      shown to interact with HIV-1 IN and facilitate lentiviral vector integration. 
      These findings reveal a new function of TTRAP and expand our understanding of the 
      cellular response to HIV infection. The interaction between TTRAP and HIV-1 IN 
      may be useful in designing new anti-viral strategies as well as for improving the 
      efficiency of lentiviral-vector-mediated gene delivery.
FAU - Zhang, Jian-qi
AU  - Zhang JQ
AD  - State Key Laboratory of Genetic Engineering, Institute of Genetics, School of 
      Life Sciences, Fudan University, China.
FAU - Wang, Jing-jing
AU  - Wang JJ
FAU - Li, Wen-juan
AU  - Li WJ
FAU - Huang, Lu
AU  - Huang L
FAU - Tian, Ling
AU  - Tian L
FAU - Xue, Jing-lun
AU  - Xue JL
FAU - Chen, Jin-zhong
AU  - Chen JZ
FAU - Jia, William
AU  - Jia W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090704
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Transcription Factors)
RN  - EC 2.7.7.- (HIV Integrase)
RN  - EC 3.1.4.- (Phosphoric Diester Hydrolases)
RN  - EC 3.1.4.- (TDP2 protein, human)
RN  - YY6481J2FF (p31 integrase protein, Human immunodeficiency virus 1)
SB  - IM
MH  - Cell Line
MH  - DNA-Binding Proteins
MH  - Gene Knockdown Techniques
MH  - HIV Integrase/*metabolism
MH  - HIV-1/enzymology/genetics/*physiology
MH  - Humans
MH  - Nuclear Proteins/genetics/*metabolism
MH  - Phosphoric Diester Hydrolases
MH  - Transcription Factors/genetics/*metabolism
MH  - *Virus Integration
EDAT- 2009/07/08 09:00
MHDA- 2009/08/21 09:00
CRDT- 2009/07/08 09:00
PHST- 2009/06/16 00:00 [received]
PHST- 2009/06/26 00:00 [accepted]
PHST- 2009/07/08 09:00 [entrez]
PHST- 2009/07/08 09:00 [pubmed]
PHST- 2009/08/21 09:00 [medline]
AID - S0006-291X(09)01309-6 [pii]
AID - 10.1016/j.bbrc.2009.06.153 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2009 Sep 18;387(2):256-60. doi: 
      10.1016/j.bbrc.2009.06.153. Epub 2009 Jul 4.