PMID- 19580837
OWN - NLM
STAT- MEDLINE
DCOM- 20091211
LR  - 20220311
IS  - 1879-1166 (Electronic)
IS  - 0198-8859 (Linking)
VI  - 70
IP  - 10
DP  - 2009 Oct
TI  - Epitopes of human leukocyte antigen class I antibodies found in sera of normal 
      healthy males and cord blood.
PG  - 844-53
LID - 10.1016/j.humimm.2009.06.020 [doi]
AB  - This study defines 96 epitopes targeted by human leukocyte antigen (HLA) 
      antibodies reported in the sera of normal healthy males with no history of 
      deliberate alloimmunizations and in cord blood. These epitopes are accessible for 
      antibody binding on either the intact or the dissociated forms of recombinant HLA 
      class I single antigens. Sixty percent of the epitopes are accessible on 
      dissociated antigens, are defined mostly by hidden amino acids, and are 
      designated as cryptic epitopes. All 96 epitopes are located exclusively on A-, 
      B-, or C-locus antigens except for one interlocus epitope. All sera in this study 
      were tested in parallel, using single antigen beads that bear either intact or 
      dissociated HLA antigens and antibodies with nearly identical specificities were 
      identified in all tested sera. Because the specificities of these naturally 
      occurring antibodies are unavoidably detected when testing for specificities of 
      alloantibodies, it may be necessary to clearly differentiate the two forms of 
      antibody. To date, the relevance of these antibodies in transplantation is 
      unknown, but even if they are determined to be irrelevant to graft rejection, 
      awareness of the newly identified epitopes could prove useful in avoiding the 
      unnecessary exclusion of potential transplant donors.
FAU - El-Awar, Nadim
AU  - El-Awar N
AD  - One Lambda, Inc., Canoga Park, CA 90064, USA. nelawar@onelambda.com
FAU - Terasaki, Paul I
AU  - Terasaki PI
FAU - Nguyen, Anh
AU  - Nguyen A
FAU - Sasaki, Nori
AU  - Sasaki N
FAU - Morales-Buenrostro, Luis E
AU  - Morales-Buenrostro LE
FAU - Saji, Hiroh
AU  - Saji H
FAU - Maruya, Etsuko
AU  - Maruya E
FAU - Poli, Francesca
AU  - Poli F
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20090704
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (Antibodies)
RN  - 0 (Epitopes)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Isoantibodies)
SB  - IM
MH  - Antibodies/blood/chemistry/*immunology
MH  - Epitopes/chemistry/*immunology
MH  - Fetal Blood/*immunology
MH  - Graft Rejection/immunology
MH  - Histocompatibility Antigens Class I/blood/chemistry/*immunology
MH  - Humans
MH  - Isoantibodies/blood/chemistry/*immunology
MH  - Male
MH  - Protein Conformation
EDAT- 2009/07/08 09:00
MHDA- 2009/12/16 06:00
CRDT- 2009/07/08 09:00
PHST- 2008/12/08 00:00 [received]
PHST- 2009/06/20 00:00 [revised]
PHST- 2009/06/29 00:00 [accepted]
PHST- 2009/07/08 09:00 [entrez]
PHST- 2009/07/08 09:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
AID - S0198-8859(09)00168-2 [pii]
AID - 10.1016/j.humimm.2009.06.020 [doi]
PST - ppublish
SO  - Hum Immunol. 2009 Oct;70(10):844-53. doi: 10.1016/j.humimm.2009.06.020. Epub 2009 
      Jul 4.