PMID- 19582594
OWN - NLM
STAT- MEDLINE
DCOM- 20090918
LR  - 20090714
IS  - 1559-0283 (Electronic)
IS  - 1085-9195 (Linking)
VI  - 55
IP  - 1
DP  - 2009
TI  - Hepatocellular carcinoma cells deteriorate the biophysical properties of 
      dendritic cells.
PG  - 33-43
LID - 10.1007/s12013-009-9055-6 [doi]
AB  - Dendritic cells (DCs) are potent antigen-presenting cells and induce 
      antigen-specific immune responses in the organism. The dysfunction of DCs has 
      been implicated in tumor-bearing host. In order to elucidate the effects of tumor 
      microenvironment on the functions of DCs from interdisciplinary aspects, we 
      characterized the biophysical properties of DCs co-cultured with hepatocellular 
      carcinoma cells (HCC). The results showed that the biophysical characteristics of 
      immature and mature DCs were severely impaired by HCC compared with those under 
      normal conditions, including the increased osmotic fragilities, decreased cell 
      membrane fluidities, increased membrane viscoelastic properties, dysfunction and 
      increased expression of cytoskeleton protein F-actin, as well as the deteriorated 
      transendothelium migration. The impaired biophysical properties of DCs may be one 
      of many aspects of the immune escape mechanisms of tumors. These results are 
      clinically and instructionally significant with regard to how to enhance 
      efficiency of the anti-tumor therapy based on DCs.
FAU - Zeng, Zhu
AU  - Zeng Z
AD  - Department of Biophysics, Health Science Center, Peking University, Beijing 
      100191, People's Republic of China.
FAU - Yao, Weijuan
AU  - Yao W
FAU - Xu, Xiaofeng
AU  - Xu X
FAU - Xu, Guoqiang
AU  - Xu G
FAU - Long, Jinhua
AU  - Long J
FAU - Wang, Xianwei
AU  - Wang X
FAU - Wen, Zongyao
AU  - Wen Z
FAU - Chien, Shu
AU  - Chien S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090707
PL  - United States
TA  - Cell Biochem Biophys
JT  - Cell biochemistry and biophysics
JID - 9701934
RN  - 0 (Actins)
SB  - IM
MH  - Actins/metabolism
MH  - Biophysical Phenomena
MH  - Carcinoma, Hepatocellular/*immunology/therapy
MH  - Cell Movement
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Dendritic Cells/cytology/*immunology/transplantation
MH  - Endothelial Cells/cytology
MH  - Humans
MH  - Liver Neoplasms/*immunology/therapy
MH  - *Tumor Escape
EDAT- 2009/07/08 09:00
MHDA- 2009/09/19 06:00
CRDT- 2009/07/08 09:00
PHST- 2009/02/21 00:00 [received]
PHST- 2009/06/18 00:00 [accepted]
PHST- 2009/07/08 09:00 [entrez]
PHST- 2009/07/08 09:00 [pubmed]
PHST- 2009/09/19 06:00 [medline]
AID - 10.1007/s12013-009-9055-6 [doi]
PST - ppublish
SO  - Cell Biochem Biophys. 2009;55(1):33-43. doi: 10.1007/s12013-009-9055-6. Epub 2009 
      Jul 7.