PMID- 19583844
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20091211
LR  - 20211020
IS  - 1471-2326 (Electronic)
IS  - 1471-2326 (Linking)
VI  - 9
DP  - 2009 Jul 7
TI  - Application of tri-colour, dual fusion fluorescence in situ hybridization (FISH) 
      system for the characterization of BCR-ABL1 fusion in chronic myelogenous 
      leukaemia (CML) and residual disease monitoring.
PG  - 4
LID - 10.1186/1471-2326-9-4 [doi]
AB  - BACKGROUND: We studied the application of the BCR-ABL1 + 9q34 tri-colour dual 
      fusion fluorescence in situ hybridization (FISH) system in the characterization 
      of fusion signal pattern and the monitoring of residual disease in chronic 
      myelogenous leukaemia (CML). The signal constellation on metaphases with the 
      tri-colour dual fusion system was defined. The knowledge of various signal 
      patterns obtained from the different genetic rearrangements was further applied 
      to the analysis of hybridization signals on interphase nuclei. METHODS: BCR-ABL1 
      dual colour, dual fusion FISH (D-FISH) was performed on diagnostic samples of 22 
      CML patients. The tri-colour FISH system was performed on cases that showed 
      aberrant signal patterns other than the classical 1 green (G) 1 orange (O) 2 
      fusions (F). Using the aqua band-pass filter, random signal overlap in interphase 
      nuclei would be indicated by the presence of an aqua signal (ASS1), while genuine 
      fusion was represented by the absence of the ASS1 signal. RESULTS: Using the 
      D-FISH system, the signal patterns could be categorized into 4 groups: group 1 (n 
      = 17) showed the classical 1G1O2F; group 2 (n = 2) showed 2G1O1F indicating ABL1 
      deletion; group 3 (n = 1) showed 1G2O1F indicating BCR deletion; group 4 (n = 2) 
      with 1G1O1F indicating reciprocal ABL1-BCR deletion. The tri-colour dual fusion 
      system correlated with the D-FISH system for cases with der(9) deletion. The 
      added aqua-labelled ASS1 probe was useful in differentiating random signal 
      overlap from genuine BCR-ABL1 fusion in the interphase cells (group 4). 
      CONCLUSION: Although the D-FISH probe was valuable in establishing the different 
      patterns of aberrant signals and monitoring patients with the classic 2-fusion 
      signals in CML, the tri-colour dual fusion probe should be used for patients with 
      der(9) deletion to monitor response to treatment.
FAU - Siu, Lisa Lp
AU  - Siu LL
AD  - Department of Pathology, Queen Elizabeth Hospital, Hong Kong SAR, PR China.
FAU - Ma, Edmond Sk
AU  - Ma ES
AD  - Division of Molecular Pathology, Department of Pathology, Hong Kong Sanatorium & 
      Hospital, Hong Kong SAR, PR China.
FAU - Wong, Wai Shan
AU  - Wong WS
AD  - Department of Pathology, Queen Elizabeth Hospital, Hong Kong SAR, PR China.
FAU - Chan, Man Hong
AU  - Chan MH
AD  - Department of Medicine, Queen Elizabeth Hospital, Hong Kong SAR, PR China.
FAU - Wong, Kit Fai
AU  - Wong KF
AD  - Department of Pathology, Queen Elizabeth Hospital, Hong Kong SAR, PR China.
LA  - eng
PT  - Journal Article
DEP - 20090707
PL  - England
TA  - BMC Blood Disord
JT  - BMC blood disorders
JID - 100968550
PMC - PMC2715371
EDAT- 2009/07/09 09:00
MHDA- 2009/07/09 09:01
PMCR- 2009/07/07
CRDT- 2009/07/09 09:00
PHST- 2009/01/15 00:00 [received]
PHST- 2009/07/07 00:00 [accepted]
PHST- 2009/07/09 09:00 [entrez]
PHST- 2009/07/09 09:00 [pubmed]
PHST- 2009/07/09 09:01 [medline]
PHST- 2009/07/07 00:00 [pmc-release]
AID - 1471-2326-9-4 [pii]
AID - 10.1186/1471-2326-9-4 [doi]
PST - epublish
SO  - BMC Blood Disord. 2009 Jul 7;9:4. doi: 10.1186/1471-2326-9-4.