PMID- 19583858
OWN - NLM
STAT- MEDLINE
DCOM- 20090803
LR  - 20211020
IS  - 1471-2156 (Electronic)
IS  - 1471-2156 (Linking)
VI  - 10
DP  - 2009 Jul 7
TI  - Comparative chromosome mapping of repetitive sequences. Implications for genomic 
      evolution in the fish, Hoplias malabaricus.
PG  - 34
LID - 10.1186/1471-2156-10-34 [doi]
AB  - BACKGROUND: Seven karyomorphs of the fish, Hoplias malabaricus (A-G) were 
      previously included in two major groups, Group I (A, B, C, D) and Group II (E, F, 
      G), based on their similar karyotype structure. In this paper, karyomorphs from 
      Group I were analyzed by means of distinct chromosomal markers, including 
      silver-stained nucleolar organizer regions (Ag-NORs) and chromosomal location of 
      repetitive sequences (18S and 5S rDNA, and satellite 5SHindIII-DNA), through 
      fluorescence in situ hybridization (FISH), in order to evaluate the evolutionary 
      relationships among them. RESULTS: The results showed that several chromosomal 
      markers had conserved location in the four karyomorphs. In addition, some other 
      markers were only conserved in corresponding chromosomes of karyomorphs A-B and 
      C-D. These data therefore reinforced and confirmed the proposed grouping of 
      karyomorphs A-D in Group I and highlight a closer relationship between 
      karyomorphs A-B and C-D. Moreover, the mapping pattern of some markers on some 
      autosomes and on the chromosomes of the XY and X1X2Y systems provided new 
      evidence concerning the possible origin of the sex chromosomes. CONCLUSION: The 
      in situ investigation of repetitive DNA sequences adds new informative characters 
      useful in comparative genomics at chromosomal level and provides insights into 
      the evolutionary relationships among Hoplias malabaricus karyomorphs.
FAU - Cioffi, Marcelo B
AU  - Cioffi MB
AD  - Universidade Federal de Sao Carlos, Departamento de Genetica e Evolucao, Sao 
      Carlos, SP, Brazil. mbello.ufscar@gmail.com
FAU - Martins, Cesar
AU  - Martins C
FAU - Bertollo, Luiz A C
AU  - Bertollo LA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090707
PL  - England
TA  - BMC Genet
JT  - BMC genetics
JID - 100966978
RN  - 0 (DNA, Ribosomal)
RN  - 0 (DNA, Satellite)
RN  - 0 (Genetic Markers)
RN  - 0 (RNA, Ribosomal, 18S)
RN  - EC 2.1.1.- (DNA modification methylase HindIII)
RN  - EC 2.1.1.72 (Site-Specific DNA-Methyltransferase (Adenine-Specific))
SB  - IM
EIN - BMC Genet. 2013;14:24
MH  - Animals
MH  - Chromosome Mapping/*methods
MH  - DNA, Ribosomal/genetics
MH  - DNA, Satellite/genetics
MH  - *Evolution, Molecular
MH  - Fishes/*genetics
MH  - Genetic Markers
MH  - Genome
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Nucleolus Organizer Region/genetics
MH  - RNA, Ribosomal, 18S/genetics
MH  - *Repetitive Sequences, Nucleic Acid
MH  - Sequence Analysis, DNA
MH  - Site-Specific DNA-Methyltransferase (Adenine-Specific)/genetics
PMC - PMC2713275
EDAT- 2009/07/09 09:00
MHDA- 2009/08/04 09:00
PMCR- 2009/07/07
CRDT- 2009/07/09 09:00
PHST- 2009/03/18 00:00 [received]
PHST- 2009/07/07 00:00 [accepted]
PHST- 2009/07/09 09:00 [entrez]
PHST- 2009/07/09 09:00 [pubmed]
PHST- 2009/08/04 09:00 [medline]
PHST- 2009/07/07 00:00 [pmc-release]
AID - 1471-2156-10-34 [pii]
AID - 10.1186/1471-2156-10-34 [doi]
PST - epublish
SO  - BMC Genet. 2009 Jul 7;10:34. doi: 10.1186/1471-2156-10-34.