PMID- 19585553
OWN - NLM
STAT- MEDLINE
DCOM- 20091104
LR  - 20171116
IS  - 1542-9741 (Electronic)
IS  - 1542-9733 (Linking)
VI  - 86
IP  - 4
DP  - 2009 Aug
TI  - Reproductive toxicity and pharmacokinetics of di-n-butyl phthalate (DBP) 
      following dietary exposure of pregnant rats.
PG  - 345-54
LID - 10.1002/bdrb.20199 [doi]
AB  - Most rodent developmental toxicity studies of dibutylphthalate (DBP) have relied 
      on bolus gavage dosing. This study characterized the developmental toxicity of 
      dietary DBP. Pregnant CD rats were given nominal doses of 0, 100, or 500 mg 
      DBP/kg/day in diet (actual intake 0, 112, and 582 mg/kg/day) from gestational day 
      (GD) 12 through the morning of GD 19. Rats were killed 4 or 24 hr thereafter. DBP 
      dietary exposure resulted in significant dose-dependent reductions in testicular 
      mRNA concentration of scavenger receptor class B, member 1; steroidogenic acute 
      regulatory protein; cytochrome P450, family 11, subfamily a, polypeptide 1; and 
      cytochrome P450 family 17, subfamily a, polypeptide 1. These effects were most 
      pronounced 4 hr after the end of exposure. Testicular testosterone was reduced 24 
      hr post-exposure in both DBP dose groups and 4 hr after termination of the 500-mg 
      DBP/kg/day exposure. Maternal exposure to 500 mg DBP/kg/day induced a significant 
      reduction in male offspring's anogenital distance indicating in utero disruption 
      of androgen function. Leydig cell aggregates, increased cord diameters, and 
      multinucleated gonocytes were present in DBP-treated rats. Monobutyl phthalate, 
      the developmentally toxic metabolite of DBP, and its glucuronide conjugate were 
      found in maternal and fetal plasma, amniotic fluid, and maternal urine. Our 
      results, when compared to previously conducted gavage studies, indicate that 
      approximately equal doses of oral DBP exposure of pregnant rats, from diet or 
      gavage, result in similar responses in male offspring.
CI  - Copyright (c) 2009 Wiley-Liss, Inc.
FAU - Struve, Melanie F
AU  - Struve MF
AD  - CIIT at The Hamner Institutes for Health Sciences, 6 Davis Drive, P.O. Box 12137, 
      Research Triangle Park, NC 27709-2137, USA.
FAU - Gaido, Kevin W
AU  - Gaido KW
FAU - Hensley, Janan B
AU  - Hensley JB
FAU - Lehmann, Kim P
AU  - Lehmann KP
FAU - Ross, Susan M
AU  - Ross SM
FAU - Sochaski, Mark A
AU  - Sochaski MA
FAU - Willson, Gabrielle A
AU  - Willson GA
FAU - Dorman, David C
AU  - Dorman DC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Birth Defects Res B Dev Reprod Toxicol
JT  - Birth defects research. Part B, Developmental and reproductive toxicology
JID - 101155115
RN  - 0 (Androgen Antagonists)
RN  - 0 (Glucuronides)
RN  - 0 (Phthalic Acids)
RN  - 0 (Scarb1 protein, rat)
RN  - 0 (Scavenger Receptors, Class B)
RN  - 0 (Steroids)
RN  - 2286E5R2KE (Dibutyl Phthalate)
RN  - 3XMK78S47O (Testosterone)
RN  - ZI46LWZ45G (monobutyl phthalate)
SB  - IM
MH  - Administration, Oral
MH  - Amniotic Fluid/chemistry
MH  - Androgen Antagonists/administration & dosage/pharmacokinetics/*toxicity
MH  - Animals
MH  - Biotransformation
MH  - Body Weight/drug effects
MH  - Dibutyl Phthalate/administration & dosage/pharmacokinetics/*toxicity
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Gas Chromatography-Mass Spectrometry
MH  - Gene Expression Regulation, Developmental/drug effects
MH  - Genitalia, Male/drug effects/embryology/pathology
MH  - Gestational Age
MH  - Glucuronides/analysis/blood/pharmacokinetics/urine
MH  - Male
MH  - Phthalic Acids/*analysis/blood/urine
MH  - Pregnancy
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Scavenger Receptors, Class B/drug effects
MH  - Steroids/biosynthesis
MH  - Testis/drug effects/embryology/metabolism/pathology
MH  - Testosterone/biosynthesis
EDAT- 2009/07/09 09:00
MHDA- 2009/11/05 06:00
CRDT- 2009/07/09 09:00
PHST- 2009/07/09 09:00 [entrez]
PHST- 2009/07/09 09:00 [pubmed]
PHST- 2009/11/05 06:00 [medline]
AID - 10.1002/bdrb.20199 [doi]
PST - ppublish
SO  - Birth Defects Res B Dev Reprod Toxicol. 2009 Aug;86(4):345-54. doi: 
      10.1002/bdrb.20199.