PMID- 19586896 OWN - NLM STAT- MEDLINE DCOM- 20091117 LR - 20190923 IS - 0253-9772 (Print) IS - 0253-9772 (Linking) VI - 31 IP - 4 DP - 2009 Apr TI - [Analysis of the chromosomes of S. bicolor x S. propinquum and S. dochna x S. propinquum by FISH]. PG - 420-5 AB - By means of fluorescence in situ hybridization (FISH), we located and analyzed the sites of the 45S rDNA on the F1 hybrids of S. bicolor x S. propinquum and S. dochnaxS.propinquum. Two signals of 45S rDNA were mapped on the mitotic metaphase chromosomes in the F1 hybrids, respectively, and one signal was present on a bivalent during meiotic of synaptene, diakinesis, and metaphase I. We inferred that the two mitotic chromosomes carrying 45S rDNA were homologous pairs. Considering the signals of 45S rDNA location during meiotic process, chromosome pairing of these two F1 hybrids was normal with an average pairing configuration of 2n=2x=20 (10 II). Our results indicated that 45S rDNA could provide a landmark for identification of individual chromosome during meiosis indirectly. FAU - Xie, Li AU - Xie L AD - College of Life Science, Guangxi Normal University, Guilin 541004, China. guilinxieli@163.com FAU - Han, Yong-Hua AU - Han YH FAU - Li, Dong-Yu AU - Li DY FAU - Zeng, Yan-Hua AU - Zeng YH LA - chi PT - English Abstract PT - Journal Article PL - China TA - Yi Chuan JT - Yi chuan = Hereditas JID - 9436478 RN - 0 (DNA, Plant) RN - 0 (DNA, Ribosomal) SB - IM MH - Chimera/*genetics MH - Chromosomes, Plant/*genetics MH - DNA, Plant/genetics MH - DNA, Ribosomal/genetics MH - In Situ Hybridization, Fluorescence/*methods MH - Sorghum/*genetics EDAT- 2009/07/10 09:00 MHDA- 2009/11/18 06:00 CRDT- 2009/07/10 09:00 PHST- 2009/07/10 09:00 [entrez] PHST- 2009/07/10 09:00 [pubmed] PHST- 2009/11/18 06:00 [medline] AID - 0253-9772(2009)04-420-6 [pii] AID - 10.3724/sp.j.1005.2009.00420 [doi] PST - ppublish SO - Yi Chuan. 2009 Apr;31(4):420-5. doi: 10.3724/sp.j.1005.2009.00420.