PMID- 19591984
OWN - NLM
STAT- MEDLINE
DCOM- 20100525
LR  - 20220310
IS  - 1556-5653 (Electronic)
IS  - 0015-0282 (Linking)
VI  - 93
IP  - 7
DP  - 2010 May 1
TI  - Double probing individual human spermatozoa: aniline blue staining for persistent 
      histones and fluorescence in situ hybridization for aneuploidies.
PG  - 2255-61
LID - 10.1016/j.fertnstert.2009.05.033 [doi]
AB  - OBJECTIVE: To study the potential relationship between two sperm nuclear 
      attributes: persistence of histones and occurrence of chromosomal aneuploidies. 
      DESIGN: The two variables were examined by double probing of the same 
      spermatozoa. SETTING: Academic Andrology Laboratory. PATIENT(S): Semen samples 
      subjected for analyses were examined. INTERVENTION(S): We studied >58,000 
      spermatozoa, in seven men, first with aniline blue histone staining, graded as 
      light (mature sperm), intermediate (moderately immature), and dark (severely 
      arrested maturation). After recording the staining patterns and destaining, the 
      same spermatozoa were subjected to fluorescence in situ hybridization (FISH), 
      using centrometric X, Y, and 17 chromosome probes. MAIN OUTCOME MEASURE(S): 
      Proportions of sperm with light, intermediate, and dark staining were assessed, 
      and ploidy of these sperm was evaluated. RESULT(S): The aneuploidy frequencies in 
      intermediate versus light (mature) spermatozoa were increased four- to sixfold. 
      In addition, aneuploidy frequencies and proportions of intermediate sperm were 
      related. There was no FISH signal detectable in the darkly stained, severely 
      arrested mature sperm. CONCLUSION(S): The data suggest that in sperm with 
      arrested maturity and DNA fragmentation, the binding of FISH probes is 
      diminished. DNA damage is further aggravated by the decondensation and 
      denaturation steps of FISH. Thus, there is a strong likelihood that in 
      oligozoospermic men, with a higher proportion of sperm with arrested maturation, 
      the sperm disomy frequencies are historically underestimated.
CI  - Copyright 2010 American Society for Reproductive Medicine. Published by Elsevier 
      Inc. All rights reserved.
FAU - Ovari, Laszlo
AU  - Ovari L
AD  - Yale Sperm Physiology Laboratory, Yale University, School of Medicine, New Haven, 
      Connecticut 06510, USA.
FAU - Sati, Leyla
AU  - Sati L
FAU - Stronk, Jill
AU  - Stronk J
FAU - Borsos, Antal
AU  - Borsos A
FAU - Ward, David C
AU  - Ward DC
FAU - Huszar, Gabor
AU  - Huszar G
LA  - eng
GR  - HD-19505/HD/NICHD NIH HHS/United States
GR  - OH-04061/OH/NIOSH CDC HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20090709
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
RN  - 0 (Aniline Compounds)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Histones)
RN  - 8004-91-9 (aniline blue)
SB  - IM
MH  - *Aneuploidy
MH  - Aniline Compounds/*pharmacology
MH  - DNA Damage/physiology
MH  - Fluorescent Dyes/pharmacology
MH  - Histones/chemistry/drug effects/*metabolism
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Infertility, Male/diagnosis/genetics/metabolism/pathology
MH  - Karyotyping/methods
MH  - Male
MH  - Semen Analysis/methods
MH  - Spermatozoa/*cytology/metabolism
MH  - Staining and Labeling/*methods
EDAT- 2009/07/14 09:00
MHDA- 2010/05/26 06:00
CRDT- 2009/07/14 09:00
PHST- 2009/03/09 00:00 [received]
PHST- 2009/05/12 00:00 [revised]
PHST- 2009/05/12 00:00 [accepted]
PHST- 2009/07/14 09:00 [entrez]
PHST- 2009/07/14 09:00 [pubmed]
PHST- 2010/05/26 06:00 [medline]
AID - S0015-0282(09)01109-1 [pii]
AID - 10.1016/j.fertnstert.2009.05.033 [doi]
PST - ppublish
SO  - Fertil Steril. 2010 May 1;93(7):2255-61. doi: 10.1016/j.fertnstert.2009.05.033. 
      Epub 2009 Jul 9.