PMID- 19592231
OWN - NLM
STAT- MEDLINE
DCOM- 20091130
LR  - 20191210
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 25
IP  - 1
DP  - 2009 Sep 15
TI  - Aptamer-Au NPs conjugates-enhanced SPR sensing for the ultrasensitive sandwich 
      immunoassay.
PG  - 124-9
LID - 10.1016/j.bios.2009.06.016 [doi]
AB  - The goal of this work is to explore the amplification effect of aptamer-gold 
      nanoparticles (Au NPs) conjugates for ultrasensitive detection of large 
      biomolecules by surface plasmon resonance (SPR). A novel sandwich immunoassay is 
      designed to demonstrate the amplification effect of aptamer-Au NPs conjugates by 
      using human immunoglobulin E (IgE) as model analyte. Human IgE, captured by 
      immobilized goat anti-human IgE on SPR gold film, is sensitively detected by SPR 
      spectroscopy with a lowest detection limit of 1 ng/ml after anti-human IgE 
      aptamer-Au NPs conjugates is used as amplification reagent. Meanwhile, the 
      non-specific adsorption of aptamer-Au NPs conjugates on goat anti-human IgE is 
      confirmed by SPR spectroscopy and then it is minimized by treating aptamer-Au NPs 
      conjugates with 6-mercaptohexan-1-ol (MCH). These results confirm that aptamer-Au 
      NPs conjugates is a powerful sandwich element and an excellent amplification 
      reagent for SPR-based sandwich immunoassay.
FAU - Wang, Jianlong
AU  - Wang J
AD  - Department of Chemical Engineering, Worcester Polytechnic Institute, 100 
      Institute Road, Worcester, MA 01609, United States.
FAU - Munir, Ahsan
AU  - Munir A
FAU - Li, Zhonghong
AU  - Li Z
FAU - Zhou, H Susan
AU  - Zhou HS
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20090617
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Aptamers, Nucleotide)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - Aptamers, Nucleotide/*chemistry/immunology
MH  - Gold/*chemistry
MH  - Humans
MH  - Immunoassay/*methods
MH  - Immunoglobulin E/*analysis/immunology
MH  - Metal Nanoparticles/*chemistry
MH  - Sensitivity and Specificity
MH  - Surface Plasmon Resonance/*methods
EDAT- 2009/07/14 09:00
MHDA- 2009/12/16 06:00
CRDT- 2009/07/14 09:00
PHST- 2009/03/25 00:00 [received]
PHST- 2009/06/04 00:00 [revised]
PHST- 2009/06/08 00:00 [accepted]
PHST- 2009/07/14 09:00 [entrez]
PHST- 2009/07/14 09:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
AID - S0956-5663(09)00329-7 [pii]
AID - 10.1016/j.bios.2009.06.016 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2009 Sep 15;25(1):124-9. doi: 10.1016/j.bios.2009.06.016. 
      Epub 2009 Jun 17.