PMID- 19594397
OWN - NLM
STAT- MEDLINE
DCOM- 20090916
LR  - 20090714
IS  - 1557-8976 (Electronic)
IS  - 0882-8245 (Linking)
VI  - 22
IP  - 4
DP  - 2009 Jul
TI  - Changes in lymphocyte subsets and cytokines during European porcine reproductive 
      and respiratory syndrome: increased expression of IL-12 and IL-10 and 
      proliferation of CD4(-)CD8(high).
PG  - 261-71
LID - 10.1089/vim.2009.0003 [doi]
AB  - The changes in peripheral blood mononuclear cells (PBMCs) have been studied in 
      several reports in an attempt to determine the immune response against porcine 
      reproductive and respiratory syndrome virus (PRRSV) infection. However, how these 
      changes are evoked after PRRSV infection has not yet been clarified. The aim of 
      this study was to analyze the changes seen in lymphocyte subsets and 
      immunomodulatory cytokine expression in pigs after an acute experimental 
      infection with a European PRRSV field isolate. Pigs were inoculated 
      intramuscularly with PRRSV field isolate 2982. Samples from blood, medial 
      retropharyngeal and tracheobronchial lymph nodes, and spleen were collected at 
      different time points for flow cytometry studies and for cytokine expression by 
      ELISA. CD21(+) cell counts increased in PBMCs and tracheobronchial lymph node 
      cells from 17 to 24 dpi, coinciding with an increase in PRRSV-specific antibody 
      titer in blood. CD3(+) T-cell counts increased mainly due to an enhancement of 
      CD4(-)CD8(high) and CD4(+)CD8(+) T cells. CD4(-)CD8(low) T cells were decreased 
      in all organs studied, whereas CD4(+)CD8(-) T cells decreased only in the spleen. 
      The drop in viremia correlated with an enhancement of CD4(-)CD8(high) T cells, 
      and with a higher expression of interleukin-10 (IL-10) and interleukin-12 p40 
      (IL-12 p40). No efficient interferon-gamma (IFN-gamma) response was detected 
      during the acute phase of the infection, and the expression of interferon-alpha 
      (IFN-alpha) was late and reached its maximum expression once the viremia 
      decreased. These results point to IL-10 and IL-12 as cytokines that might play a 
      significant role in the PRRSV immune response, as may CD4(-)CD8(high) T cells.
FAU - Gomez-Laguna, Jaime
AU  - Gomez-Laguna J
AD  - Department of Anatomy and Comparative Pathology, Faculty of Veterinary Medicine, 
      Cordoba University, Campus de Rabanales, Edificio de Sanidad Animal, Cordoba, 
      Spain. v92golaj@uco.es
FAU - Salguero, Francisco J
AU  - Salguero FJ
FAU - De Marco, Mar Fernandez
AU  - De Marco MF
FAU - Pallares, Francisco J
AU  - Pallares FJ
FAU - Bernabe, Antonio
AU  - Bernabe A
FAU - Carrasco, Librado
AU  - Carrasco L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Viral Immunol
JT  - Viral immunology
JID - 8801552
RN  - 0 (Interferon-alpha)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - B-Lymphocytes/immunology/metabolism
MH  - CD8-Positive T-Lymphocytes/immunology/*metabolism
MH  - Europe
MH  - Interferon-alpha/biosynthesis/immunology
MH  - Interferon-gamma/biosynthesis/immunology
MH  - Interleukin-10/*biosynthesis/immunology
MH  - Interleukin-12/*biosynthesis/immunology
MH  - Leukocytes, Mononuclear/immunology/metabolism
MH  - Lymphocyte Activation
MH  - Lymphocyte Count
MH  - *Lymphocyte Subsets/immunology/metabolism
MH  - Male
MH  - Porcine Reproductive and Respiratory 
      Syndrome/*immunology/*metabolism/physiopathology
MH  - Swine
MH  - Viremia
EDAT- 2009/07/15 09:00
MHDA- 2009/09/17 06:00
CRDT- 2009/07/15 09:00
PHST- 2009/07/15 09:00 [entrez]
PHST- 2009/07/15 09:00 [pubmed]
PHST- 2009/09/17 06:00 [medline]
AID - 10.1089/vim.2009.0003 [doi]
PST - ppublish
SO  - Viral Immunol. 2009 Jul;22(4):261-71. doi: 10.1089/vim.2009.0003.