PMID- 19595750 OWN - NLM STAT- MEDLINE DCOM- 20090928 LR - 20151119 IS - 1879-3169 (Electronic) IS - 0378-4274 (Linking) VI - 190 IP - 2 DP - 2009 Oct 28 TI - Pipping success and liver mRNA expression in chicken embryos exposed in ovo to C8 and C11 perfluorinated carboxylic acids and C10 perfluorinated sulfonate. PG - 134-9 LID - 10.1016/j.toxlet.2009.07.004 [doi] AB - Several perfluoroalkyl compounds (PFCs) are ubiquitous environmental contaminants that can biomagnify in species at high trophic levels including wild birds. Perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) have been detected in wild birds and are known to reduce hatching success of laboratory-exposed chicken embryos at environmentally relevant concentrations. Limited toxicity data are available regarding avian exposure to PFCs of chain lengths greater than C(8), which are of increasing environmental relevance following the recent phase-out of PFOS and PFOA. In this study, linear PFOA, perfluoroundecanoic acid (PFUdA) and perfluorodecane sulfonate (PFDS) were injected into the air cell of white leghorn chicken eggs (Gallus gallus domesticus) prior to incubation to determine effects on embryo pipping success. Furthermore, mRNA expression of key genes involved in pathways implicated in PFC toxicity was monitored in liver tissue. PFOA, PFUdA or PFDS had no effect on embryonic pipping success at concentrations up to 10 microg/g. All PFCs accumulated in the liver to concentrations greater than the initial whole-egg concentration as determined by HPLC/MS/MS. Hepatic accumulation was highest for PFOA (4.5 times) compared to PFUdA and PFDS. Cytochrome P450 1A4 and liver fatty acid binding protein mRNA expression increased after exposure to PFUdA but was only statistically significant at 10 microg/g; several orders of magnitude higher than levels found in wild bird eggs. Based on the present results for white leghorn chickens, current environmental concentrations of PFOA, PFUdA and PFDS are unlikely to affect the hatching success of wild birds. FAU - O'Brien, Jason M AU - O'Brien JM AD - Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, 20 Marie Curie Street, Ottawa, Ontario K1N 6N5, Canada. FAU - Crump, Doug AU - Crump D FAU - Mundy, Lukas J AU - Mundy LJ FAU - Chu, Shaogang AU - Chu S FAU - McLaren, Kristina K AU - McLaren KK FAU - Vongphachan, Viengtha AU - Vongphachan V FAU - Letcher, Robert J AU - Letcher RJ FAU - Kennedy, Sean W AU - Kennedy SW LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20090710 PL - Netherlands TA - Toxicol Lett JT - Toxicology letters JID - 7709027 RN - 0 (Avian Proteins) RN - 0 (Caprylates) RN - 0 (Carboxylic Acids) RN - 0 (Fluorocarbons) RN - 0 (RNA, Messenger) RN - 0 (Sulfonic Acids) RN - 947VD76D3L (perfluorooctanoic acid) RN - EC 1.14.14.1 (Aryl Hydrocarbon Hydroxylases) RN - EC 1.14.14.1 (CYP1A4 protein, Gallus gallus) RN - EC 1.14.14.1 (cytochrome P-450 CYP1A5) SB - IM MH - Animals MH - Aryl Hydrocarbon Hydroxylases/biosynthesis/genetics MH - Avian Proteins/biosynthesis/genetics MH - Caprylates/pharmacokinetics/*toxicity MH - Carboxylic Acids/pharmacokinetics/*toxicity MH - Chick Embryo MH - Chromatography, High Pressure Liquid MH - Embryonic Development/*drug effects MH - Fluorocarbons/pharmacokinetics/*toxicity MH - Liver/*metabolism MH - Mass Spectrometry MH - RNA, Messenger/*biosynthesis MH - Reverse Transcriptase Polymerase Chain Reaction MH - Sex Determination Processes MH - Sulfonic Acids/pharmacokinetics/*toxicity EDAT- 2009/07/15 09:00 MHDA- 2009/09/29 06:00 CRDT- 2009/07/15 09:00 PHST- 2009/06/10 00:00 [received] PHST- 2009/06/30 00:00 [revised] PHST- 2009/07/01 00:00 [accepted] PHST- 2009/07/15 09:00 [entrez] PHST- 2009/07/15 09:00 [pubmed] PHST- 2009/09/29 06:00 [medline] AID - S0378-4274(09)01206-5 [pii] AID - 10.1016/j.toxlet.2009.07.004 [doi] PST - ppublish SO - Toxicol Lett. 2009 Oct 28;190(2):134-9. doi: 10.1016/j.toxlet.2009.07.004. Epub 2009 Jul 10.