PMID- 19596016
OWN - NLM
STAT- MEDLINE
DCOM- 20090923
LR  - 20160512
IS  - 1089-8638 (Electronic)
IS  - 0022-2836 (Linking)
VI  - 392
IP  - 1
DP  - 2009 Sep 11
TI  - Crystal structure of the TNF-alpha-Inducing protein (Tipalpha) from Helicobacter 
      pylori: Insights into Its DNA-binding activity.
PG  - 191-7
LID - 10.1016/j.jmb.2009.07.010 [doi]
AB  - Helicobacter pylori infection is one of the highest risk factors for 
      gastroduodenal diseases including gastric cancer. Tumor necrosis factor-alpha 
      (TNF-alpha) is one of the essential cytokines for tumor promotion, and thus, an 
      H. pylori protein that induces TNF-alpha is believed to play a significant role 
      in gastric cancer development in humans. The HP0596 gene product of H. pylori 
      strain 26695 was identified as the TNF-alpha-inducing protein (Tipalpha). 
      Tipalpha is secreted from H. pylori as dimers and enters the gastric cells. It 
      was shown to have a DNA-binding activity. Here, we have determined the crystal 
      structure of Tipalpha from H. pylori. Its monomer consists of two structural 
      domains ("mixed domain" and "helical domain"). Tipalpha exists as a dimer in the 
      crystal, and the dimeric structure represents a novel scaffold for DNA binding. A 
      positively charged surface patch formed across the two monomers of the Tipalpha 
      dimer by the loop between helices alpha1 and alpha2 may be important in DNA 
      binding.
FAU - Jang, Jun Young
AU  - Jang JY
AD  - Department of Chemistry, Seoul National University, Korea.
FAU - Yoon, Hye-Jin
AU  - Yoon HJ
FAU - Yoon, Ji Young
AU  - Yoon JY
FAU - Kim, Hyoun Sook
AU  - Kim HS
FAU - Lee, Sang Jae
AU  - Lee SJ
FAU - Kim, Kyoung Hoon
AU  - Kim KH
FAU - Kim, Do Jin
AU  - Kim DJ
FAU - Jang, Soonmin
AU  - Jang S
FAU - Han, Byeong-Gu
AU  - Han BG
FAU - Lee, Byung Il
AU  - Lee BI
FAU - Suh, Se Won
AU  - Suh SW
LA  - eng
SI  - PDB/3GIO
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090710
PL  - Netherlands
TA  - J Mol Biol
JT  - Journal of molecular biology
JID - 2985088R
RN  - 0 (Bacterial Proteins)
RN  - 0 (Tipalpha protein, Helicobacter pylori)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Amino Acid Sequence
MH  - Bacterial Proteins/*chemistry/metabolism
MH  - Crystallography, X-Ray
MH  - DNA/metabolism
MH  - Dimerization
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Protein Binding
MH  - Protein Structure, Quaternary
MH  - Protein Structure, Tertiary
MH  - Sequence Alignment
EDAT- 2009/07/15 09:00
MHDA- 2009/09/24 06:00
CRDT- 2009/07/15 09:00
PHST- 2009/03/06 00:00 [received]
PHST- 2009/07/01 00:00 [revised]
PHST- 2009/07/06 00:00 [accepted]
PHST- 2009/07/15 09:00 [entrez]
PHST- 2009/07/15 09:00 [pubmed]
PHST- 2009/09/24 06:00 [medline]
AID - S0022-2836(09)00836-5 [pii]
AID - 10.1016/j.jmb.2009.07.010 [doi]
PST - ppublish
SO  - J Mol Biol. 2009 Sep 11;392(1):191-7. doi: 10.1016/j.jmb.2009.07.010. Epub 2009 
      Jul 10.