PMID- 19596783
OWN - NLM
STAT- MEDLINE
DCOM- 20090915
LR  - 20211020
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 29
IP  - 18
DP  - 2009 Sep
TI  - The multiple endocrine neoplasia type 1 (MEN1) tumor suppressor regulates 
      peroxisome proliferator-activated receptor gamma-dependent adipocyte 
      differentiation.
PG  - 5060-9
LID - 10.1128/MCB.01001-08 [doi]
AB  - Menin, the product of the MEN1 (multiple endocrine neoplasia type 1) tumor 
      suppressor gene, is involved in activation of gene transcription as part of an 
      MLL1 (mixed-lineage leukemia 1)/MLL2 (KMT2A/B)-containing protein complex which 
      harbors methyltransferase activity for lysine 4 of histone H3 (H3K4). As MEN1 
      patients frequently develop lipomas and peroxisome proliferator-activated 
      receptor gamma (PPARgamma) is expressed in several MEN1-related tumor types, we 
      investigated regulation of PPARgamma activity by menin. We found that menin is 
      required for adipocyte differentiation of murine 3T3-L1 cells and 
      PPARgamma-expressing mouse embryonic fibroblasts. Menin augments PPARgamma target 
      gene expression through recruitment of H3K4 methyltransferase activity. Menin 
      interacts directly with the activation function 2 transcription activation domain 
      of PPARgamma in a ligand-independent fashion. Ligand-dependent coactivation, 
      however, is dependent on the LXXLL motif of menin and the intact helix 12 of 
      PPARgamma. We propose that menin is an important factor in PPARgamma-mediated 
      adipogenesis and that loss of PPARgamma function may contribute to lipoma 
      development in MEN1 patients.
FAU - Dreijerink, Koen M A
AU  - Dreijerink KM
AD  - University Medical Center Utrecht, Department of Physiological Chemistry, 
      Utrecht, The Netherlands.
FAU - Varier, Radhika A
AU  - Varier RA
FAU - van Beekum, Olivier
AU  - van Beekum O
FAU - Jeninga, Ellen H
AU  - Jeninga EH
FAU - Hoppener, Jo W M
AU  - Hoppener JW
FAU - Lips, Cornelis J M
AU  - Lips CJ
FAU - Kummer, J Alain
AU  - Kummer JA
FAU - Kalkhoven, Eric
AU  - Kalkhoven E
FAU - Timmers, H T Marc
AU  - Timmers HT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090713
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (FABP4 protein, human)
RN  - 0 (Fatty Acid-Binding Proteins)
RN  - 0 (Histones)
RN  - 0 (Ligands)
RN  - 0 (MEN1 protein, human)
RN  - 0 (Men1 protein, mouse)
RN  - 0 (PPAR gamma)
RN  - 0 (Proto-Oncogene Proteins)
RN  - K3Z4F929H6 (Lysine)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/*cytology/*metabolism
MH  - Animals
MH  - *Cell Differentiation
MH  - Fatty Acid-Binding Proteins/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Gene Knockdown Techniques
MH  - Histones/metabolism
MH  - Humans
MH  - Ligands
MH  - Lysine/metabolism
MH  - Methylation
MH  - Mice
MH  - PPAR gamma/chemistry/*metabolism
MH  - Protein Binding
MH  - Protein Structure, Tertiary
MH  - Proto-Oncogene Proteins/*metabolism
MH  - Transcription, Genetic
PMC - PMC2738285
EDAT- 2009/07/15 09:00
MHDA- 2009/09/16 06:00
PMCR- 2010/03/01
CRDT- 2009/07/15 09:00
PHST- 2009/07/15 09:00 [entrez]
PHST- 2009/07/15 09:00 [pubmed]
PHST- 2009/09/16 06:00 [medline]
PHST- 2010/03/01 00:00 [pmc-release]
AID - MCB.01001-08 [pii]
AID - 1001-08 [pii]
AID - 10.1128/MCB.01001-08 [doi]
PST - ppublish
SO  - Mol Cell Biol. 2009 Sep;29(18):5060-9. doi: 10.1128/MCB.01001-08. Epub 2009 Jul 
      13.