PMID- 19596831
OWN - NLM
STAT- MEDLINE
DCOM- 20091008
LR  - 20131121
IS  - 1535-3699 (Electronic)
IS  - 1535-3699 (Linking)
VI  - 234
IP  - 10
DP  - 2009 Oct
TI  - Enterovirus 71 infection of human dendritic cells.
PG  - 1166-73
LID - 10.3181/0903-RM-116 [doi]
AB  - Enterovirus 71 (EV71) causes death and long-term neurologic sequelae in hundreds 
      of thousands of young children, but its pathogenesis remains elusive. Dendritic 
      cells (DCs) play a crucial role in antiviral immunity by functioning as 
      professional antigen-presenting cells to prime T cells and by secreting cytokines 
      to modulate immune responses. Here, we show that EV71 productively infected human 
      immature DCs and expressed viral antigen in DCs. EV71 entry into DCs was 
      partially mediated by DC-SIGN. Further analyses revealed that EV71 increased the 
      viability, activation, release of cytokines, interleukin-6, interleukin-12, and 
      tumor necrosis factor-alpha in DCs. Moreover, EV71 enabled DCs to stimulate 
      T-cell proliferation. Collectively, these findings suggest that EV71 infection of 
      human DCs in vivo is very likely to elicit protective immunity, because in 
      infected mice, both T cells and IL-6 function to reduce mortality.
FAU - Lin, Yu-Wen
AU  - Lin YW
AD  - Institute of Basic Biomedical Sciences, National Cheng Kung University, Tainan, 
      Taiwan, 701, Republic of China.
FAU - Wang, Shainn-Wei
AU  - Wang SW
FAU - Tung, Yuk-Ying
AU  - Tung YY
FAU - Chen, Shun-Hua
AU  - Chen SH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090713
PL  - Switzerland
TA  - Exp Biol Med (Maywood)
JT  - Experimental biology and medicine (Maywood, N.J.)
JID - 100973463
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Cytokines)
RN  - 0 (DC-specific ICAM-3 grabbing nonintegrin)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Receptors, Cell Surface)
RN  - 11016-17-4 (Phycoerythrin)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Antibodies, Monoclonal/metabolism
MH  - Cell Adhesion Molecules/metabolism
MH  - Cell Culture Techniques
MH  - Cell Line
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Cytokines/immunology/metabolism
MH  - Dendritic Cells/immunology/*virology
MH  - Enterovirus A, Human/*immunology/metabolism
MH  - Enterovirus Infections/*immunology
MH  - Fluorescein-5-isothiocyanate/metabolism
MH  - Fluorescent Antibody Technique, Indirect
MH  - Fluorescent Dyes/metabolism
MH  - Humans
MH  - Lectins, C-Type/metabolism
MH  - Leukocytes, Mononuclear/cytology/immunology
MH  - Lymphocyte Culture Test, Mixed
MH  - Phycoerythrin/metabolism
MH  - Receptors, Cell Surface/metabolism
MH  - T-Lymphocytes/immunology/metabolism
EDAT- 2009/07/15 09:00
MHDA- 2009/10/09 06:00
CRDT- 2009/07/15 09:00
PHST- 2009/07/15 09:00 [entrez]
PHST- 2009/07/15 09:00 [pubmed]
PHST- 2009/10/09 06:00 [medline]
AID - 0903-RM-116 [pii]
AID - 10.3181/0903-RM-116 [doi]
PST - ppublish
SO  - Exp Biol Med (Maywood). 2009 Oct;234(10):1166-73. doi: 10.3181/0903-RM-116. Epub 
      2009 Jul 13.