PMID- 19601931
OWN - NLM
STAT- MEDLINE
DCOM- 20090917
LR  - 20220408
IS  - 1439-0272 (Electronic)
IS  - 0303-4569 (Linking)
VI  - 41
IP  - 4
DP  - 2009 Aug
TI  - Human sperm DNA integrity in normal and abnormal semen samples and its 
      correlation with sperm characteristics.
PG  - 207-15
LID - 10.1111/j.1439-0272.2009.00917.x [doi]
AB  - Reports indicate an increase in the incidence of DNA fragmentation in male factor 
      infertility and its role in the outcome of assisted reproductive techniques 
      (ART). However, reports are conflicting between the relationships of sperm DNA 
      integrity with conventional semen parameters. We examined the relationship 
      between conventional sperm parameters and DNA integrity using acridine orange 
      (AO) test. The study included 373 patients and 28 fertile volunteers. DNA 
      normality was compared with semen parameters between the patient and donor 
      populations. Significant correlations were noted between DNA normality and sperm 
      concentration (r = 0.18, P = 0.000), motility (r = 0.21, P = 0.0001), rapid 
      motility (0.19, P = 0.000), normal morphology by World Health Organization (r = 
      0.15, P = 0.019) and head defects (r = -0.15, P = 0.023). A significant 
      difference was noted in AO levels between donors and patients with 
      asthenozoospermia (P = 0.002) and oligoasthenozoospermia (P = 0.001). A 
      significant difference in DNA integrity was noted in samples having <30% and >30% 
      normal morphology. A wide range of % DNA normality was observed in the patient 
      group. Sperm assessment for DNA status using AO is reliable and shows good 
      correlation with sperm count, motility and morphology. Assessment of sperm DNA 
      status with AO staining may be helpful prior to ART.
FAU - Varghese, A C
AU  - Varghese AC
AD  - Fertility Clinic and IVF Department, AMRI Medical Centre, Kolkata, India.
FAU - Bragais, F M
AU  - Bragais FM
FAU - Mukhopadhyay, D
AU  - Mukhopadhyay D
FAU - Kundu, S
AU  - Kundu S
FAU - Pal, M
AU  - Pal M
FAU - Bhattacharyya, A K
AU  - Bhattacharyya AK
FAU - Agarwal, A
AU  - Agarwal A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Andrologia
JT  - Andrologia
JID - 0423506
RN  - 9007-49-2 (DNA)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - DNA/*genetics
MH  - *DNA Fragmentation
MH  - Humans
MH  - Infertility, Male/genetics
MH  - Male
MH  - *Semen Analysis
MH  - Sperm Count
MH  - Sperm Motility/genetics
MH  - Spermatozoa/*physiology
MH  - Tissue Donors
EDAT- 2009/07/16 09:00
MHDA- 2009/09/18 06:00
CRDT- 2009/07/16 09:00
PHST- 2009/07/16 09:00 [entrez]
PHST- 2009/07/16 09:00 [pubmed]
PHST- 2009/09/18 06:00 [medline]
AID - AND917 [pii]
AID - 10.1111/j.1439-0272.2009.00917.x [doi]
PST - ppublish
SO  - Andrologia. 2009 Aug;41(4):207-15. doi: 10.1111/j.1439-0272.2009.00917.x.