PMID- 19604518
OWN - NLM
STAT- MEDLINE
DCOM- 20091116
LR  - 20221207
IS  - 1532-8600 (Electronic)
IS  - 0026-0495 (Linking)
VI  - 58
IP  - 11
DP  - 2009 Nov
TI  - FORT and FORD: two simple and rapid assays in the evaluation of oxidative stress 
      in patients with type 2 diabetes mellitus.
PG  - 1657-62
LID - 10.1016/j.metabol.2009.05.022 [doi]
AB  - The aim of the study was to evaluate the levels of free oxygen radicals and free 
      oxygen radicals defense in patients with newly diagnosed type 2 diabetes mellitus 
      (T2DM). The disease seems to be involved strongly in the production of reactive 
      oxygen species. Forty-five patients with newly diagnosed T2DM and 20 apparently 
      healthy individuals (control group) were included in the study. Reactive oxygen 
      species were determined using the free oxygen radicals (FORT) test, which is 
      based on the Fenton reaction. In this method, the hydroperoxides reacted with the 
      transition metal ions liberated from the proteins and were converted to alkoxy 
      and peroxy radicals. The radical species produced by the reaction, which are 
      directly proportional to the quantity of lipid peroxides, interact with an 
      additive that forms a radical molecule. Similarly, the free oxygen radicals 
      defense (FORD) test uses preformed stable and colored radicals and determines the 
      decrease in absorbance that is proportional to the blood antioxidant 
      concentration. We found that (a) FORT levels were increased in diabetic patients 
      (2.86 +/- 0.56 mmol/L H(2)O(2)) compared with controls (1.87 +/- 0.26 mmol/L 
      H(2)O(2)) (P < .0001) and (b) FORD levels were lower in diabetic patients (1.23 
      +/- 0.18 mmol/L Trolox) compared with controls (1.34 +/- 0.14 mmol/L Trolox) (P < 
      .01). The intraassay and interassay coefficients of variation were 3.7% and 6.2%, 
      respectively, for FORT and 4.2% and 6.6%, respectively, for FORD. Determination 
      of free oxygen radicals and free oxygen radicals defense seems to play an 
      important role in the generation and evaluation of oxidative stress, an imbalance 
      between oxidants and antioxidants that can lead to oxidative damage and is 
      involved in the pathogenesis of several diseases, such as T2DM.
FAU - Pavlatou, Maria G
AU  - Pavlatou MG
AD  - Department of Clinical Biochemistry, Aghia Sophia Children's Hospital, 115 27 
      Athens, Greece.
FAU - Papastamataki, Maria
AU  - Papastamataki M
FAU - Apostolakou, Filia
AU  - Apostolakou F
FAU - Papassotiriou, Ioannis
AU  - Papassotiriou I
FAU - Tentolouris, Nicholas
AU  - Tentolouris N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090715
PL  - United States
TA  - Metabolism
JT  - Metabolism: clinical and experimental
JID - 0375267
RN  - 0 (Glycated Hemoglobin A)
RN  - 0 (Insulin)
RN  - 0 (Lipids)
RN  - 0 (Reactive Oxygen Species)
RN  - 9007-41-4 (C-Reactive Protein)
RN  - 9007-73-2 (Ferritins)
SB  - IM
MH  - Aged
MH  - Algorithms
MH  - Body Mass Index
MH  - C-Reactive Protein/metabolism
MH  - Chromatography, High Pressure Liquid
MH  - Diabetes Mellitus, Type 2/*metabolism
MH  - Female
MH  - Ferritins/blood
MH  - Glycated Hemoglobin/metabolism
MH  - Humans
MH  - Insulin/blood
MH  - Lipids/blood
MH  - Male
MH  - Middle Aged
MH  - Oxidation-Reduction
MH  - Oxidative Stress/*physiology
MH  - Reactive Oxygen Species/*blood
EDAT- 2009/07/17 09:00
MHDA- 2009/11/17 06:00
CRDT- 2009/07/17 09:00
PHST- 2009/02/14 00:00 [received]
PHST- 2009/05/12 00:00 [revised]
PHST- 2009/05/27 00:00 [accepted]
PHST- 2009/07/17 09:00 [entrez]
PHST- 2009/07/17 09:00 [pubmed]
PHST- 2009/11/17 06:00 [medline]
AID - S0026-0495(09)00222-4 [pii]
AID - 10.1016/j.metabol.2009.05.022 [doi]
PST - ppublish
SO  - Metabolism. 2009 Nov;58(11):1657-62. doi: 10.1016/j.metabol.2009.05.022. Epub 
      2009 Jul 15.