PMID- 19605354
OWN - NLM
STAT- MEDLINE
DCOM- 20091021
LR  - 20240425
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 284
IP  - 40
DP  - 2009 Oct 2
TI  - Germ line-governed recognition of a cancer epitope by an immunodominant human 
      T-cell receptor.
PG  - 27281-9
LID - 10.1074/jbc.M109.022509 [doi]
AB  - CD8(+) T-cells specific for MART-1-(26-35), a dominant melanoma epitope 
      restricted by human leukocyte antigen (HLA)-A*0201, are exceptionally common in 
      the naive T-cell repertoire. Remarkably, the TRAV12-2 gene is used to encode the 
      T-cell receptor alpha (TCRalpha) chain in >87% of these T-cells. Here, the 
      molecular basis for this genetic bias is revealed from the structural and 
      thermodynamic properties of an archetypal TRAV12-2-encoded TCR complexed to the 
      clinically relevant heteroclitic peptide, ELAGIGILTV, bound to HLA-A*0201 
      (A2-ELA). Unusually, the TRAV12-2 germ line-encoded regions of the TCR dominate 
      the major atomic contacts with the peptide at the TCR/A2-ELA interface. This 
      "innate" pattern of antigen recognition probably explains the unique 
      characteristics and extraordinary frequencies of CD8(+) T-cell responses to this 
      epitope.
FAU - Cole, David K
AU  - Cole DK
AD  - Department of Infection, Immunity, and Biochemistry, Henry Wellcome Building, 
      Cardiff University School of Medicine, Heath Park, Cardiff CF14 4XN, United 
      Kingdom.
FAU - Yuan, Fang
AU  - Yuan F
FAU - Rizkallah, Pierre J
AU  - Rizkallah PJ
FAU - Miles, John J
AU  - Miles JJ
FAU - Gostick, Emma
AU  - Gostick E
FAU - Price, David A
AU  - Price DA
FAU - Gao, George F
AU  - Gao GF
FAU - Jakobsen, Bent K
AU  - Jakobsen BK
FAU - Sewell, Andrew K
AU  - Sewell AK
LA  - eng
SI  - PDB/3HG1
GR  - BB/H001085/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090715
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-A*02:01 antigen)
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (Immunodominant Epitopes)
RN  - 0 (MART-1 Antigen)
RN  - 0 (MLANA protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Receptors, Antigen, T-Cell)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antigens, Neoplasm/chemistry
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Germ Cells/*immunology
MH  - HLA-A Antigens/immunology
MH  - HLA-A2 Antigen
MH  - Humans
MH  - Immunity, Innate
MH  - Immunodominant Epitopes/chemistry/*immunology
MH  - MART-1 Antigen
MH  - Melanoma/*immunology
MH  - Models, Molecular
MH  - Neoplasm Proteins/chemistry
MH  - Protein Conformation
MH  - Receptors, Antigen, T-Cell/chemistry/genetics/*immunology
MH  - Solubility
MH  - Substrate Specificity
MH  - Thermodynamics
PMC - PMC2785656
EDAT- 2009/07/17 09:00
MHDA- 2009/10/22 06:00
PMCR- 2010/10/02
CRDT- 2009/07/17 09:00
PHST- 2009/07/17 09:00 [entrez]
PHST- 2009/07/17 09:00 [pubmed]
PHST- 2009/10/22 06:00 [medline]
PHST- 2010/10/02 00:00 [pmc-release]
AID - S0021-9258(20)38397-6 [pii]
AID - M109.022509 [pii]
AID - 10.1074/jbc.M109.022509 [doi]
PST - ppublish
SO  - J Biol Chem. 2009 Oct 2;284(40):27281-9. doi: 10.1074/jbc.M109.022509. Epub 2009 
      Jul 15.