PMID- 19606012
OWN - NLM
STAT- MEDLINE
DCOM- 20090917
LR  - 20090826
IS  - 1532-0979 (Electronic)
IS  - 0147-5185 (Linking)
VI  - 33
IP  - 9
DP  - 2009 Sep
TI  - Distinguishing epithelioid blue nevus from blue nevus-like cutaneous melanoma 
      metastasis using fluorescence in situ hybridization.
PG  - 1396-400
LID - 10.1097/PAS.0b013e3181a92cbc [doi]
AB  - Blue nevus (BN)-like cutaneous melanoma metastasis is a well-recognized variant 
      of melanoma metastasis. These lesions may clinically and histologically simulate 
      benign blue nevi. The histologic changes may be indistinguishable from 
      conventional blue nevi or epithelioid blue nevi (EBN), a benign dermal-based 
      melanocytic neoplasm with epithelioid morphology and heavily pigmented cytoplasm. 
      Distinguishing BN-like cutaneous melanoma metastasis from benign conventional or 
      EBN is important for staging and treatment. We evaluated a fluorescence in situ 
      hybridization (FISH) assay using probes targeting 6p25 (RREB1), 6q23 (MYB), 11q13 
      (CCND1), and centromere 6 (Cep6) with previously determined criteria, to 
      distinguish EBN and BN-like melanoma metastasis. Ten BN-like cutaneous melanoma 
      metastatic lesions and 10 EBN were blindly evaluated with the above mentioned 
      FISH probes. FISH enumeration and criteria for diagnosis of melanoma was as 
      previously described. Nine of 10 BN-like cutaneous metastatic lesions showed 
      significant aberrations and met previously established criteria for melanoma. 
      None of the EBN cases showed evidence of significant copy number changes or met 
      FISH criteria for a diagnosis of melanoma. FISH is an important diagnostic 
      adjunct for melanocytic neoplasms. In this study, we show that a FISH assay 
      targeting 6p25, 6q23, 11q13, and centromere 6 can distinguish EBN from BN-like 
      metastatic melanoma with high accuracy. The test and the parameters previously 
      established can perform with high sensitivity and specificity when dealing with 
      this differential diagnosis.
FAU - Pouryazdanparast, Pedram
AU  - Pouryazdanparast P
AD  - Department of Pathology, Feinberg School of Medicine, Northwestern University, 
      Chicago, IL 60611, USA.
FAU - Newman, Marissa
AU  - Newman M
FAU - Mafee, Mariam
AU  - Mafee M
FAU - Haghighat, Zahra
AU  - Haghighat Z
FAU - Guitart, Joan
AU  - Guitart J
FAU - Gerami, Pedram
AU  - Gerami P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Surg Pathol
JT  - The American journal of surgical pathology
JID - 7707904
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosome Aberrations
MH  - DNA, Neoplasm/analysis
MH  - Diagnosis, Differential
MH  - Epithelioid Cells/*pathology
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Melanoma/*diagnosis/genetics/secondary
MH  - Middle Aged
MH  - Nevus, Blue/*diagnosis/genetics
MH  - Predictive Value of Tests
MH  - Skin Neoplasms/*diagnosis/genetics
MH  - Young Adult
EDAT- 2009/07/17 09:00
MHDA- 2009/09/18 06:00
CRDT- 2009/07/17 09:00
PHST- 2009/07/17 09:00 [entrez]
PHST- 2009/07/17 09:00 [pubmed]
PHST- 2009/09/18 06:00 [medline]
AID - 10.1097/PAS.0b013e3181a92cbc [doi]
PST - ppublish
SO  - Am J Surg Pathol. 2009 Sep;33(9):1396-400. doi: 10.1097/PAS.0b013e3181a92cbc.