PMID- 19608218
OWN - NLM
STAT- MEDLINE
DCOM- 20100218
LR  - 20121115
IS  - 1879-2472 (Electronic)
IS  - 0049-3848 (Linking)
VI  - 124
IP  - 5
DP  - 2009 Nov
TI  - Effect of tissue inhibitor of matrix metalloproteinases-1 on in vitro and in vivo 
      adipocyte differentiation.
PG  - 578-83
LID - 10.1016/j.thromres.2009.06.020 [doi]
AB  - Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) play a role 
      in development of obesity by contributing to adipogenesis, angiogenesis and 
      extracellular matrix degradation. We have evaluated a potential functional role 
      of TIMP-1, which inhibits most MMPs, in early stages of in vitro and in vivo 
      adipogenesis. Overexpression of human TIMP-1 (hTIMP-1) in 3T3-F442A preadipocytes 
      resulted in a somewhat slower differentiation into mature adipocytes, without 
      affecting the total extent of differentiation. Local overexpression by injection 
      of 3T3-F442A preadipocytes expressing hTIMP-1 in the back of NUDE mice kept on 
      high fat diet (HFD) for 4 weeks, had no effect on de novo formed fat pad mass. 
      The fat pads formed from the hTIMP-1 expressing cells did show a significantly 
      larger blood vessel size as compared to control cells (57 + or - 4.8 microm(2) vs 
      38 + or - 1.4 microm(2), p=0.0017). No effect was observed on blood vessel 
      density or on adipocyte size or density. Thus, local hTIMP-1 overexpression did 
      not significantly affect early stages of adipogenesis as evaluated from the 
      extent of in vitro adipocyte differentiation or of in vivo de novo adipose tissue 
      formation.
FAU - Scroyen, Ilse
AU  - Scroyen I
AD  - Center for Molecular and Vascular Biology, KU Leuven, Leuven, Belgium.
FAU - Cosemans, Leentje
AU  - Cosemans L
FAU - Lijnen, H Roger
AU  - Lijnen HR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090716
PL  - United States
TA  - Thromb Res
JT  - Thrombosis research
JID - 0326377
RN  - 0 (Matrix Metalloproteinase Inhibitors)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - 3T3 Cells
MH  - Adipocytes/*cytology/enzymology/*metabolism
MH  - Adipogenesis/drug effects/genetics
MH  - Animals
MH  - Cell Differentiation/physiology
MH  - Humans
MH  - Male
MH  - Matrix Metalloproteinase 2/metabolism
MH  - Matrix Metalloproteinase 9/metabolism
MH  - Matrix Metalloproteinase Inhibitors
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tissue Inhibitor of Metalloproteinase-1/*biosynthesis/genetics
MH  - Transfection
EDAT- 2009/07/18 09:00
MHDA- 2010/02/19 06:00
CRDT- 2009/07/18 09:00
PHST- 2009/04/01 00:00 [received]
PHST- 2009/06/16 00:00 [revised]
PHST- 2009/06/19 00:00 [accepted]
PHST- 2009/07/18 09:00 [entrez]
PHST- 2009/07/18 09:00 [pubmed]
PHST- 2010/02/19 06:00 [medline]
AID - S0049-3848(09)00306-5 [pii]
AID - 10.1016/j.thromres.2009.06.020 [doi]
PST - ppublish
SO  - Thromb Res. 2009 Nov;124(5):578-83. doi: 10.1016/j.thromres.2009.06.020. Epub 
      2009 Jul 16.