PMID- 19610027
OWN - NLM
STAT- MEDLINE
DCOM- 20100413
LR  - 20100226
IS  - 1099-1573 (Electronic)
IS  - 0951-418X (Linking)
VI  - 24
IP  - 3
DP  - 2010 Mar
TI  - Effects of Bupleurum falcatum and its combination with an angiotensin II receptor 
      blocker on cytokine and chemokine expression in human mesangial cells.
PG  - 339-43
LID - 10.1002/ptr.2936 [doi]
AB  - This study aimed to investigate the inhibitory effect of Bupleurum falcatum and 
      its combination with angiotensin II receptor blocker (ARB) on cytokine and 
      chemokine production in cultured human mesangial cells. Human mesangial cells 
      were isolated and cultured in Dulbecco's modified Eagle's medium culture medium. 
      Bupleurum falcatum, ARB, and the combination of the two were added to human 
      mesangial cells. Cytokine and chemokine levels were analysed using an 
      enzyme-linked immunosorbent assay. There were no significant differences in the 
      expression of IL-1ss, IL-2 or TNF-a between controls and the experimental groups. 
      However, IL-11 and monocyte chemoattractant protein-1 (MCP-1) levels were 
      significantly reduced in response to ARB, Bupleurum falcatum, or their 
      combination when compared with controls. IL-8 expression was reduced 
      significantly only in cells treated with ARB. Both Bupleurum falcatum and ARB 
      treatments alone reduced the cytokine concentration, but there was not a stronger 
      reduction when the two drugs were combined. It was shown that Bupleurum falcatum 
      inhibited cytokine production in human mesangial cells. However, there were no 
      additive effects on the suppression of cytokine production when Bupleurum 
      falcatum was combined with ARB. Further studies are needed to elucidate the 
      renoprotective effects of Bupleurum falcatum.
CI  - (c) 2009 John Wiley & Sons, Ltd.
FAU - Cho, Byoung-Soo
AU  - Cho BS
AD  - Department of Pediatrics, East West Kidney Diseases Research Institute, Kyung Hee 
      University, Seoul, Korea.
FAU - Kim, Sung-Do
AU  - Kim SD
FAU - Park, Jae-Kyung
AU  - Park JK
FAU - Chung, Joo-Ho
AU  - Chung JH
FAU - Hong, Mee-Suk
AU  - Hong MS
FAU - Lee, Byung-Cheol
AU  - Lee BC
FAU - Ihm, Chun-Gyoo
AU  - Ihm CG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Phytother Res
JT  - Phytotherapy research : PTR
JID - 8904486
RN  - 0 (Angiotensin II Type 1 Receptor Blockers)
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Angiotensin II Type 1 Receptor Blockers/*pharmacology
MH  - Bupleurum/*chemistry
MH  - Cells, Cultured
MH  - Chemokines/*metabolism
MH  - Cytokines/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Herb-Drug Interactions
MH  - Humans
MH  - Mesangial Cells/*drug effects/metabolism
MH  - Plant Extracts/*pharmacology
EDAT- 2009/07/18 09:00
MHDA- 2010/04/14 06:00
CRDT- 2009/07/18 09:00
PHST- 2009/07/18 09:00 [entrez]
PHST- 2009/07/18 09:00 [pubmed]
PHST- 2010/04/14 06:00 [medline]
AID - 10.1002/ptr.2936 [doi]
PST - ppublish
SO  - Phytother Res. 2010 Mar;24(3):339-43. doi: 10.1002/ptr.2936.