PMID- 19610040
OWN - NLM
STAT- MEDLINE
DCOM- 20100413
LR  - 20221207
IS  - 1099-1573 (Electronic)
IS  - 0951-418X (Linking)
VI  - 24
IP  - 3
DP  - 2010 Mar
TI  - Betulinic acid protects against ischemia/reperfusion-induced renal damage and 
      inhibits leukocyte apoptosis.
PG  - 325-32
LID - 10.1002/ptr.2929 [doi]
AB  - The possible protective effect of betulinic acid on renal ischemia/reperfusion 
      (I/R) injury was studied. Wistar Albino rats were unilaterally nephrectomized and 
      subjected to 45 min of renal pedicle occlusion followed by 6 h of reperfusion. 
      Betulinic acid (250 mg/kg, i.p.) or saline was administered at 30 min prior to 
      ischemia and immediately before the reperfusion. Creatinine, blood urea nitrogen 
      (BUN), lactate dehydrogenase (LDH) and TNF-alpha as well as the oxidative burst 
      of neutrophil and leukocyte apoptosis were assayed in blood samples. 
      Malondialdehyde (MDA), glutathione (GSH) levels, Na(+), K(+)-ATPase and 
      myeloperoxidase (MPO) activities were determined in kidney tissue which was also 
      analysed microscopically. I/R caused significant increases in blood creatinine, 
      BUN, LDH and TNF-alpha. In the kidney samples of the I/R group, MDA levels and 
      MPO activity were increased significantly, however, GSH levels and Na(+), 
      K(+)-ATPase activity were decreased. Betulinic acid ameliorated the oxidative 
      burst response to both formyl-methionyl-leucyl-phenylalanine (fMLP) and phorbol 
      12-myristate 13-acetate (PMA) stimuli, normalized the apoptotic response and most 
      of the biochemical indices as well as histopathological alterations induced by 
      I/R. In conclusion, these data suggest that betulinic acid attenuates I/R-induced 
      oxidant responses, improved microscopic damage and renal function by regulating 
      the apoptotic function of leukocytes and inhibiting neutrophil infiltration.
CI  - (c) 2009 John Wiley & Sons, Ltd.
FAU - Eksioglu-Demiralp, Emel
AU  - Eksioglu-Demiralp E
AD  - Marmara University, School of Medicine, Department of Hematology and Immunology, 
      Istanbul, Turkey. demiralp@marmara.edu.tr
FAU - Kardas, E Riza
AU  - Kardas ER
FAU - Ozgul, Seckin
AU  - Ozgul S
FAU - Yagci, Tayfur
AU  - Yagci T
FAU - Bilgin, Huseyin
AU  - Bilgin H
FAU - Sehirli, Ozer
AU  - Sehirli O
FAU - Ercan, Feriha
AU  - Ercan F
FAU - Sener, Goksel
AU  - Sener G
LA  - eng
PT  - Journal Article
PL  - England
TA  - Phytother Res
JT  - Phytotherapy research : PTR
JID - 8904486
RN  - 0 (Pentacyclic Triterpenes)
RN  - 0 (Triterpenes)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - EC 7.2.2.13 (Sodium-Potassium-Exchanging ATPase)
RN  - 4G6A18707N (Betulinic Acid)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Kidney/drug effects/*pathology
MH  - Leukocytes/*drug effects
MH  - Male
MH  - Oxidative Stress/drug effects
MH  - Pentacyclic Triterpenes
MH  - Peroxidase/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Reperfusion Injury/*prevention & control
MH  - Respiratory Burst
MH  - Sodium-Potassium-Exchanging ATPase/metabolism
MH  - Triterpenes/*pharmacology
MH  - Betulinic Acid
EDAT- 2009/07/18 09:00
MHDA- 2010/04/14 06:00
CRDT- 2009/07/18 09:00
PHST- 2009/07/18 09:00 [entrez]
PHST- 2009/07/18 09:00 [pubmed]
PHST- 2010/04/14 06:00 [medline]
AID - 10.1002/ptr.2929 [doi]
PST - ppublish
SO  - Phytother Res. 2010 Mar;24(3):325-32. doi: 10.1002/ptr.2929.