PMID- 19615517
OWN - NLM
STAT- MEDLINE
DCOM- 20090925
LR  - 20220321
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 79
IP  - 4
DP  - 2009 Sep 15
TI  - Simple sequential injection analysis system for rapid determination of 
      microalbuminuria.
PG  - 1104-10
LID - 10.1016/j.talanta.2009.02.020 [doi]
AB  - A simple, specific and sensitive sequential injection analysis (SIA) system based 
      on non-immunoassay fluorescent detection has been developed for the determination 
      of urinary albumin. The specific binding of the dye Albumin Blue 580 (AB 580) to 
      albumin in urine generated high emission fluorescent signals. The excitation and 
      emission wavelengths were set at 590 and 610 nm, respectively. The analytical 
      range was obtained from 1 to 100 mg L(-1), with a detection limit of 0.3 mg L(-1) 
      (S/N=3). The SIA system gave high precision with relative standard deviations 
      (R.S.D.s) of 0.9% and 1.4% when evaluated with 15 and 100 mg L(-1) albumin 
      (n=15), respectively. The method exhibited good reproducibility, as assessed by 
      performing four analytical curves on different days, and intra-run CVs (2.3-3.3%) 
      and inter-run CVs (3.8%) were obtained. Rapid operation was achieved with a 
      sample throughput of 37 h(-1). This method was successfully applied to the 
      determination of urinary albumin, and the method was highly correlated with the 
      immunoturbidimetric method (r(2)=0.965; n=72).
FAU - Laiwattanapaisal, Wanida
AU  - Laiwattanapaisal W
AD  - Department of Clinical Chemistry, Faculty of Allied Health Sciences, 
      Chulalongkorn University, Bangkok 10330, Thailand. wanida.k@chula.ac.th
FAU - Kunanuvat, Usakorn
AU  - Kunanuvat U
FAU - Intharachuti, Wichabhorn
AU  - Intharachuti W
FAU - Chinvongamorn, Chakorn
AU  - Chinvongamorn C
FAU - Hannongbua, Supot
AU  - Hannongbua S
FAU - Chailapakul, Orawon
AU  - Chailapakul O
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090220
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Albumin Blue 580)
RN  - 0 (Albumins)
RN  - 0 (Buffers)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Nitriles)
SB  - IM
MH  - Albumins/chemistry
MH  - Albuminuria/diagnosis/*urine
MH  - Buffers
MH  - Flow Injection Analysis/*methods
MH  - Fluorescence
MH  - Fluorescent Dyes/chemistry
MH  - Humans
MH  - Nitriles/chemistry
MH  - Reproducibility of Results
MH  - Time Factors
MH  - Urinalysis/*methods
EDAT- 2009/07/21 09:00
MHDA- 2009/09/26 06:00
CRDT- 2009/07/21 09:00
PHST- 2008/11/18 00:00 [received]
PHST- 2009/02/04 00:00 [revised]
PHST- 2009/02/07 00:00 [accepted]
PHST- 2009/07/21 09:00 [entrez]
PHST- 2009/07/21 09:00 [pubmed]
PHST- 2009/09/26 06:00 [medline]
AID - S0039-9140(09)00145-3 [pii]
AID - 10.1016/j.talanta.2009.02.020 [doi]
PST - ppublish
SO  - Talanta. 2009 Sep 15;79(4):1104-10. doi: 10.1016/j.talanta.2009.02.020. Epub 2009 
      Feb 20.