PMID- 19616275
OWN - NLM
STAT- MEDLINE
DCOM- 20091021
LR  - 20161125
IS  - 1879-1298 (Electronic)
IS  - 0045-6535 (Linking)
VI  - 76
IP  - 11
DP  - 2009 Sep
TI  - In vitro assessment of AhR-mediated activities of TCDD in mixture with humic 
      substances.
PG  - 1505-8
LID - 10.1016/j.chemosphere.2009.06.042 [doi]
AB  - Humic substances (HS) are ubiquitous natural products of decomposition of dead 
      organic matter. HS is present in most freshwaters at concentrations ranging from 
      0.5 to 50mg L(-1). Organic carbon can represent 20% dry weight of sediments. 
      Recently, the interaction of dissolved HS with the aryl hydrocarbon receptor 
      (AhR) has been demonstrated. The AhR is a cytosolic receptor to which persistent 
      organic pollutants (POPs) can bind and many of their toxic effects are mediated 
      through interactions with this receptor. We describe in vitro effects (using 
      H4IIE-luc cells) of binary mixtures of various HS with 
      2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), since in contaminated environments 
      these compounds occur simultaneously. Six out of 12 HS samples activated AhR even 
      at environmentally relevant concentrations (17 mg L(-1)), but did not reach the 
      full AhR-activation even at excessive concentration. In simultaneous exposure of 
      H4IIE-luc to HS (17 mg L(-1)) and TCDD (1.2 pM) without any preincubation prior 
      to exposure, either significant additive or facilitative effects were observed. 
      No negative interactions, due to possible sorption of TCDD to HS was observed. 
      Nevertheless, if the HS-TCDD binary mixture was preincubated for 6 days prior to 
      the exposure on H4IIE-luc cells, the additive and facilitative effects were less 
      due to possible sorption of TCDD onto HS. Similar results were obtained from 
      analogous experiments with greater concentrations of both TCDD and HS.
FAU - Bittner, M
AU  - Bittner M
AD  - RECETOX, Masaryk University, 625 00 Brno, Czech Republic. bittner@recetox.muni.cz
FAU - Hilscherova, K
AU  - Hilscherova K
FAU - Giesy, J P
AU  - Giesy JP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090717
PL  - England
TA  - Chemosphere
JT  - Chemosphere
JID - 0320657
RN  - 0 (Humic Substances)
RN  - 0 (Polychlorinated Dibenzodioxins)
RN  - 0 (Receptors, Aryl Hydrocarbon)
RN  - 0 (Soil Pollutants)
SB  - IM
MH  - Animals
MH  - Cell Line, Tumor
MH  - Environmental Restoration and Remediation/methods
MH  - Humic Substances/*toxicity
MH  - Kinetics
MH  - Polychlorinated Dibenzodioxins/*toxicity
MH  - Rats
MH  - Receptors, Aryl Hydrocarbon/*metabolism
MH  - Soil Pollutants/*toxicity
EDAT- 2009/07/21 09:00
MHDA- 2009/10/22 06:00
CRDT- 2009/07/21 09:00
PHST- 2009/04/15 00:00 [received]
PHST- 2009/06/15 00:00 [revised]
PHST- 2009/06/16 00:00 [accepted]
PHST- 2009/07/21 09:00 [entrez]
PHST- 2009/07/21 09:00 [pubmed]
PHST- 2009/10/22 06:00 [medline]
AID - S0045-6535(09)00750-4 [pii]
AID - 10.1016/j.chemosphere.2009.06.042 [doi]
PST - ppublish
SO  - Chemosphere. 2009 Sep;76(11):1505-8. doi: 10.1016/j.chemosphere.2009.06.042. Epub 
      2009 Jul 17.