PMID- 19616628
OWN - NLM
STAT- MEDLINE
DCOM- 20091222
LR  - 20211028
IS  - 1095-9327 (Electronic)
IS  - 1044-7431 (Linking)
VI  - 42
IP  - 3
DP  - 2009 Nov
TI  - NG2 cells differentiate into astrocytes in cerebellar slices.
PG  - 208-18
LID - 10.1016/j.mcn.2009.07.007 [doi]
AB  - NG2-glia are an abundant population of glial cells that have been considered by 
      many to be oligodendrocyte progenitor cells (OPCs). However, growing evidence 
      suggests that NG2-glia may also be capable of differentiating into astrocytes and 
      neurons under certain conditions. Here, we have examined NG2-glia in cerebellar 
      slices, using transgenic mice in which the astroglial marker glial specific 
      protein (GFAP) drives expression of the reporter gene enhanced green fluorescent 
      protein (EGFP). Immunolabelling for NG2 shows that NG2-glia and GFAP-EGFP 
      astroglia are separate populations in most areas of the brain, although a 
      substantial population of NG2-glia in the pons also express the GFAP-EGFP 
      reporter. In the cerebellum, NG2-glia did not express EGFP, either at postnatal 
      day (P)12 or P29-30. We developed an organotypic culture of P12 cerebellar slices 
      that maintain cytoarchitectural integrity of Purkinje neurons and Bergmann glia. 
      In these cultures, BrdU labelling indicates that the majority of NG2-glia enter 
      the cell cycle within 2 days in vitro (DIV), suggesting that NG2-glia undergo a 
      'reactive' response in cerebellar cultures. After 2 DIV NG2-glia began to express 
      the astroglial reporter EGFP and in some cases the respective GFAP protein. 
      However, NG2-glia did not acquire phenotypic markers of neural stem cells or 
      neurons. The results suggest that NG2-glia are not lineage restricted OPCs and 
      are a potential source of astrocytes in the cerebellum.
FAU - Leoni, Giampaolo
AU  - Leoni G
AD  - Institute of Biology and Biomedical Sciences, School of Pharmacy and Biomedical 
      Sciences, University of Portsmouth, Portsmouth PO1 2DT, UK.
FAU - Rattray, Marcus
AU  - Rattray M
FAU - Butt, Arthur M
AU  - Butt AM
LA  - eng
GR  - BB/D012562/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090717
PL  - United States
TA  - Mol Cell Neurosci
JT  - Molecular and cellular neurosciences
JID - 9100095
RN  - 0 (Biomarkers)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Recombinant Fusion Proteins)
SB  - IM
MH  - Animals
MH  - Astrocytes/cytology/*physiology
MH  - Biomarkers/metabolism
MH  - Cell Differentiation/*physiology
MH  - Cell Lineage
MH  - Cells, Cultured
MH  - Cerebellum/*cytology
MH  - Genes, Reporter
MH  - Glial Fibrillary Acidic Protein/genetics/metabolism
MH  - Humans
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Neuroglia/cytology/*physiology
MH  - Neurons/cytology/metabolism
MH  - Recombinant Fusion Proteins/genetics/metabolism
EDAT- 2009/07/21 09:00
MHDA- 2009/12/23 06:00
CRDT- 2009/07/21 09:00
PHST- 2009/01/19 00:00 [received]
PHST- 2009/06/03 00:00 [revised]
PHST- 2009/07/03 00:00 [accepted]
PHST- 2009/07/21 09:00 [entrez]
PHST- 2009/07/21 09:00 [pubmed]
PHST- 2009/12/23 06:00 [medline]
AID - S1044-7431(09)00161-4 [pii]
AID - 10.1016/j.mcn.2009.07.007 [doi]
PST - ppublish
SO  - Mol Cell Neurosci. 2009 Nov;42(3):208-18. doi: 10.1016/j.mcn.2009.07.007. Epub 
      2009 Jul 17.