PMID- 19616936
OWN - NLM
STAT- MEDLINE
DCOM- 20091203
LR  - 20131121
IS  - 1873-2976 (Electronic)
IS  - 0960-8524 (Linking)
VI  - 100
IP  - 23
DP  - 2009 Dec
TI  - Induction of hydroxyl radical production in Trametes versicolor to degrade 
      recalcitrant chlorinated hydrocarbons.
PG  - 5757-62
LID - 10.1016/j.biortech.2009.06.078 [doi]
AB  - Extracellular hydroxyl radical ((*)OH) production via quinone redox cycling in 
      Trametes versicolor, grown in a chemically defined medium, was investigated to 
      degrade trichloroethylene (TCE), perchloroethylene (PCE), 1,2,4- and 
      1,3,5-trichlorobenzene (TCB). The activity of the enzymes catalyzing the quinone 
      redox cycle, quinone reductase and laccase, as well as the rate of (*)OH 
      production, estimated as the formation of thiobarbituric acid reactive substances 
      (TBARS) from 2-deoxyribose, increased rapidly during the first 2-3 days and then 
      remained at relatively constant levels. Under quinone redox cycling conditions, 
      TCE degradation was concomitant to TBARS production and chloride release, 
      reaching a plateau after 6 h of incubation. Similar results were obtained in PCE, 
      1,2,4- and 1,3,5-TCB time course degradation experiments. The mole balance of 
      chloride release and 1,2,4-TCB and TCE degraded suggests that these chemicals 
      were almost completely dechlorinated. Experiments using [(13)C]-TCE confirmed 
      unequivocal transformation of TCE to (13)CO(2). These results are of particular 
      interest because PCE and 1,3,5-TCB degradation in aerobic conditions has been 
      rarely reported to date in bacterial or fungal systems.
FAU - Marco-Urrea, Ernest
AU  - Marco-Urrea E
AD  - Departament d'Enginyeria Quimica and Institut de Ciencia i Tecnologia Ambiental, 
      Universitat Autonoma de Barcelona, 08193 Bellaterra, Spain. ernest.marco@uab.es
FAU - Aranda, Elisabet
AU  - Aranda E
FAU - Caminal, Gloria
AU  - Caminal G
FAU - Guillen, Francisco
AU  - Guillen F
LA  - eng
PT  - Journal Article
DEP - 20090718
PL  - England
TA  - Bioresour Technol
JT  - Bioresource technology
JID - 9889523
RN  - 0 (Chlorobenzenes)
RN  - 0 (Hydrocarbons)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - 05IQ959M1N (1,2,4-trichlorobenzene)
RN  - 290YE8AR51 (Trichloroethylene)
RN  - 2HS4M0BX3C (1,3,5-trichlorobenzene)
RN  - 3352-57-6 (Hydroxyl Radical)
RN  - 4R7X1O2820 (Chlorine)
RN  - TJ904HH8SN (Tetrachloroethylene)
SB  - IM
MH  - Biodegradation, Environmental
MH  - Biotechnology/*methods
MH  - Chlorine/*chemistry
MH  - Chlorobenzenes/chemistry
MH  - Gas Chromatography-Mass Spectrometry/methods
MH  - Hydrocarbons/*chemistry
MH  - *Hydroxyl Radical
MH  - Oxidation-Reduction
MH  - Tetrachloroethylene/chemistry
MH  - Thiobarbituric Acid Reactive Substances
MH  - Time Factors
MH  - Trametes/*metabolism
MH  - Trichloroethylene/chemistry
MH  - Water Purification/methods
EDAT- 2009/07/21 09:00
MHDA- 2009/12/16 06:00
CRDT- 2009/07/21 09:00
PHST- 2009/04/14 00:00 [received]
PHST- 2009/06/18 00:00 [revised]
PHST- 2009/06/22 00:00 [accepted]
PHST- 2009/07/21 09:00 [entrez]
PHST- 2009/07/21 09:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
AID - S0960-8524(09)00761-5 [pii]
AID - 10.1016/j.biortech.2009.06.078 [doi]
PST - ppublish
SO  - Bioresour Technol. 2009 Dec;100(23):5757-62. doi: 10.1016/j.biortech.2009.06.078. 
      Epub 2009 Jul 18.